MK5 haplodeficiency decreases collagen deposition and scar size during post-myocardial infarction wound repair.

Authors:
Sherin Ali Nawaito
Sherin Ali Nawaito
Montreal Heart Institute
Montréal | Canada
Pramod Sahadevan
Pramod Sahadevan
Université de Montréal
Montréal | Canada
Marie-Elaine Clavet-Lanthier
Marie-Elaine Clavet-Lanthier
Université de Montréal
Canada
Philippe Pouliot
Philippe Pouliot
McGill University
Canada
Fatiha Sahmi
Fatiha Sahmi
University of Montreal
Canada
Yanfen Shi
Yanfen Shi
Montreal Heart Institute and Université de Montréal
Canada
Frederic Lesage
Frederic Lesage
Rensselaer Polytechnic Institute
United States

Am J Physiol Heart Circ Physiol 2019 Jun 22;316(6):H1281-H1296. Epub 2019 Mar 22.

Department of Biochemistry and Molecular Medicine, Université de Montréal , Montreal, Quebec , Canada.

MK5 is a protein serine/threonine kinase activated by p38, ERK3, and ERK4 MAPKs. MK5 mRNA and immunoreactivity are detected in mouse cardiac fibroblasts, and MK5 haplodeficiency attenuates the increase in collagen 1-α1 mRNA evoked by pressure overload. The present study examined the effect of MK5 haplodeficiency on reparative fibrosis following myocardial infarction (MI). Twelve-week-old MK5 and wild-type littermate (MK5) mice underwent ligation of the left anterior descending coronary artery (LADL). Surviving mice were euthanized 8 or 21 days post-MI. Survival rates did not differ significantly between MK5 and MK5 mice, with rupture of the LV wall being the primary cause of death. Echocardiographic imaging revealed similar increases in LV end-diastolic diameter, myocardial performance index, and wall motion score index in LADL-MK5 and LADL-MK5 mice. Area at risk did not differ between LADL-MK5 and LADL-MK5 hearts. In contrast, infarct size, scar area, and scar collagen content were reduced in LADL-MK5 hearts. Immunohistochemical analysis of mice experiencing heart rupture revealed increased MMP-9 immunoreactivity in the infarct border zone of LADL-MK5 hearts compared with LADL-MK5. Although inflammatory cell infiltration was similar in LADL-MK5 and LADL-MK5 hearts, angiogenesis was more pronounced in the infarct border zone of LADL-MK5 mice. Characterization of ventricular fibroblasts revealed reduced motility and proliferation in fibroblasts isolated from MK5 mice compared with those from both wild-type and haplodeficient mice. siRNA-mediated knockdown of MK5 in fibroblasts from wild-type mice also impaired motility. Hence, reduced MK5 expression alters fibroblast function and scar morphology but not mortality post-MI. MK5/PRAK is a protein serine/threonine kinase activated by p38 MAPK and/or atypical MAPKs ERK3/4. MK5 haplodeficiency reduced infarct size, scar area, and scar collagen content post-myocardial infarction. Motility and proliferation were reduced in cultured MK5-null cardiac myofibroblasts.

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http://dx.doi.org/10.1152/ajpheart.00532.2017DOI Listing
June 2019
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References

(Supplied by CrossRef)
Article in J Biol Chem
Benndorf R et al.
J Biol Chem 1994

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