Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA.

Sci Rep 2019 Mar 14;9(1):4516. Epub 2019 Mar 14.

Infectious Disease and Microbiome Program, Broad Institute of Harvard and MIT, 415 Main St, Cambridge, MA, 02142, USA.

Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified.

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http://dx.doi.org/10.1038/s41598-019-40792-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418090PMC
March 2019
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