Host-antibody inductivity of virulent Entamoeba histolytica and non-virulent Entamoeba moshkovskii in a mouse model.

Authors:
Narumol Khomkhum
Narumol Khomkhum
Mahidol University
Thailand
Somphob Leetachewa
Somphob Leetachewa
Mahidol University
Thailand
Saengduen Moonsom
Saengduen Moonsom
Mahidol University
Thailand

Parasit Vectors 2019 Mar 13;12(1):101. Epub 2019 Mar 13.

Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi, Bangkok, 10400, Thailand.

Background: Despite similarities in morphology, gene and protein profiles, Entamoeba histolytica and E. moshkovskii show profound differences in pathogenicity. Entamoeba histolytica infection might result in amoebic dysentery and liver abscess, while E. moshkovskii causes only mild diarrhea. Extensive studies focus on roles of host immune responses to the pathogenic E. histolytica; however, evidence for E. moshkovskii remains scarce.

Methods: To study differences in host-antibody response profiles between E. histolytica and E. moshkovskii, mice were immunized intraperitoneally with different sets of Entamoeba trophozoites as single species, mixed species and combinations.

Results: Mice prime-immunized with E. histolytica and E. moshkovskii combination, followed by individual species, exhibited higher IgG level than the single species immunization. Mice immunized with E. moshkovskii induced significantly higher levels and long-lasting antibody responses than those challenged with E. histolytica alone. Interestingly, E. histolytica-specific anti-sera promoted the cytopathic ability of E. histolytica toward Chinese hamster ovarian (CHO) cells, but showed no effect on cell adhesion. There was no significant effect of immunized sera on cytopathic activity and adhesion of E. moshkovskii toward both CHO and human epithelial human colonic (Caco-2) cell lines. Monoclonal-antibody (mAb) characterization demonstrated that 89% of E. histolytica-specific mAbs produced from mice targeted cytoplasmic and cytoskeletal proteins, whereas 73% of E. moshkovskii-specific mAbs targeted plasma membrane proteins.

Conclusions: The present findings suggest that infection with mixed Entamoeba species or E. moshkovskii effectively induces an antibody response in mice. It also sheds light on roles of host antibody response in the pathogenic difference of E. histolytica and E. moshkovskii trophozoites, and cell surface protein modifications of the amoebic parasites to escape from host immune system.

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http://dx.doi.org/10.1186/s13071-019-3363-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416977PMC
March 2019
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References

(Supplied by CrossRef)

H Wertheim et al.
2012

A Samie et al.
2012
Article in J Travel Med.
MP Otto et al.
J Travel Med. 2013
Article in J Med Liban
S Chalhoub et al.
J Med Liban 2012
Article in Southeast Asian J Trop Med Public Health
P Viriyavejakul et al.
Southeast Asian J Trop Med Public Health 2009
Article in J Infect Dis
VQ Tran et al.
J Infect Dis 1998
Article in Parasitol Int
RM Garcia-Nieto et al.
Parasitol Int 2008
Article in Cell Microbiol
CD Huston et al.
Cell Microbiol 2000
Article in N Engl J Med
R Haque et al.
N Engl J Med 2003
Article in Parasite Immunol
IW Wilson et al.
Parasite Immunol 2012
Article in Infect Immun
Z Zhang et al.
Infect Immun 2003

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