CryoEM structure of adenovirus type 3 fibre with desmoglein 2 shows an unusual mode of receptor engagement.

Authors:
Emilie Vassal-Stermann
Emilie Vassal-Stermann
CNRS-CEA-Université Joseph Fourier
France
Gregory Effantin
Gregory Effantin
Univ. Grenoble Alpes
France
Chloe Zubieta
Chloe Zubieta
Stanford University
United States
Hongjie Wang
Hongjie Wang
Torrey Pines Institute for Molecular Studies
San Diego | United States
Andre Lieber
Andre Lieber
University of Washington
Seattle | United States
Guy Schoehn
Guy Schoehn
Université Joseph Fourier
France

Nat Commun 2019 03 12;10(1):1181. Epub 2019 Mar 12.

Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CNRS, CEA, 71 Avenue des Martyrs, 38042, Grenoble, France.

Attachment of human adenovirus (HAd) to the host cell is a critical step of infection. Initial attachment occurs via the adenoviral fibre knob protein and a cellular receptor. Here we report the cryo-electron microscopy (cryo-EM) structure of a <100 kDa non-symmetrical complex comprising the trimeric HAd type 3 fibre knob (HAd3K) and human desmoglein 2 (DSG2). The structure reveals a unique stoichiometry of 1:1 and 2:1 (DSG2: knob trimer) not previously observed for other HAd-receptor complexes. We demonstrate that mutating Asp261 in the fibre knob is sufficient to totally abolish receptor binding. These data shed new light on adenovirus infection strategies and provide insights for adenoviral vector development and structure-based design.

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Source
http://www.nature.com/articles/s41467-019-09220-y
Publisher Site
http://dx.doi.org/10.1038/s41467-019-09220-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414520PMC
March 2019
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