A prostate-specific antigen electrochemical immunosensor based on Pd NPs functionalized electroactive Co-MOF signal amplification strategy.

Biosens Bioelectron 2019 May 4;132:97-104. Epub 2019 Mar 4.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

A sandwich-type electrochemical immunosensor for prostate-specific antigen (PSA) detection was constructed. In this sensing platform, palladium nanoparticles (Pd NPs) loaded electroactive amino-zeolitic imidazolate framework-67 (Pd/NH-ZIF-67) nanocomposite is utilized as a novel redox mediator. Pd NPs, as the labeling signal molecule, owns good biocompatibility and excellent catalytic performance. Meanwhile, the electroactive ZIF-67 with lamellar structures serves not only as a carrier of Pd NPs but also as a synergistic catalyst of palladium to decompose HO. Amperometric method is used to determine the current signal generated on the electrode surface, which is conducive to achieve the quantitative determination of PSA. Under optimum conditions, the proposed immunosensor shows a wide detection range from 100 fg mL to 50 ng mL with a low detection limit of 0.03 pg mL, featuring favorable selectivity, repeatability and stability. This proposed immunosensor is expected to be a powerful tool for realizing quantitative detection of PSA and other biomarkers in actural sample analysis.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2019.02.055DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
May 2019
1 Read

Publication Analysis

Top Keywords

electrochemical immunosensor
8
prostate-specific antigen
8
proposed immunosensor
8
electroactive zif-67
4
performance electroactive
4
catalytic performance
4
excellent catalytic
4
003 pg ml featuring
4
limit 003 pg ml
4
structures serves
4
serves carrier
4
carrier nps
4
detection limit
4
biocompatibility excellent
4
lamellar structures
4
zif-67 lamellar
4
owns good
4
redox mediator
4
nps labeling
4
repeatability stability
4

Similar Publications