Cloning and characterization of the putative AFAP1-AS1 promoter region.

Authors:
Fang Wei
Fang Wei
School of Life Sciences
China
Yi He
Yi He
National Institute on Drug Abuse
China
Yan-Yan Tang
Yan-Yan Tang
University of South China
China
Li-ting Yang
Li-ting Yang
Harbin Medical University
China
Le Tang
Le Tang
Genomics Research Center (one of The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China)
Lei Shi
Lei Shi
State Key Laboratory of Lake Science and Environment
China

J Cancer 2019 29;10(5):1145-1153. Epub 2019 Jan 29.

NHC Key Laboratory of Carcinogenesis, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Actin filament-associated protein 1-antisense RNA1 (AFAP1-AS1), a cancer-related long non-coding RNA, has been found to be upregulated in multiple types of cancers. AFAP1-AS1 is important for the initiation, progression and poor prognosis of many cancers, including nasopharyngeal carcinoma (NPC). However, the mechanism underlying the regulation of AFAP1-AS1 expression is not well-understood. In our study, the potential promoter region of AFAP1-AS1 was predicted by comprehensive bioinformatics analysis. Moreover, promoter deletion analysis identified the sequence between positions -359 and -28 bp as the minimal promoter region of AFAP1-AS1. The ChIP assay results indicate that the AFAP1-AS1 promoter is responsive to the transcription factor c-Myc, which can promote high AFAP1-AS1 expression. This study is the first to clone and characterize the AFAP1-AS1 promoter region. Our findings will help to better understand the underlying mechanism of high AFAP1-AS1 expression in tumorigenesis and to develop new strategies for therapeutic high expression of AFAP1-AS1 in NPC.

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Source
http://www.jcancer.org/v10p1145.htm
Publisher Site
http://dx.doi.org/10.7150/jca.29049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400686PMC
January 2019
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