Evaluation of adenovirus amplified detection of immunochromatographic test using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.

Authors:
Tomohiro Ueno
Tomohiro Ueno
Clinical Laboratory Center
Japan
Yusuke Saeki
Yusuke Saeki
University of Tsukuba
Tsukuba | Japan
Nozomu Hanaoka
Nozomu Hanaoka
National Institute of Infectious Diseases
Japan
Tsuguto Fujimoto
Tsuguto Fujimoto
National Institute of Infectious Diseases
Japan
Eiichi Uchio
Eiichi Uchio
Fukuoka University School of Medicine

Graefes Arch Clin Exp Ophthalmol 2019 Apr 2;257(4):815-820. Epub 2019 Mar 2.

Department of Ophthalmology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan.

Background: We evaluated a novel silver amplification immunochromatography test for rapid detection of adenovirus (AdV) antigen equipped with an automated reader system using tears including conjunctival exudate in patients with adenoviral keratoconjunctivitis.

Methods: Two kinds of immunochromatographic (IC) kits, a conventional IC kit for conjunctival scrapings (control kit) and an IC kit using tears including conjunctival exudate collected by pressing a filter paper strip on the conjunctiva (test kit), were tested on 90 patients who attended Migita Eye Clinic with suspected adenoviral conjunctivitis. The results of the test kits were automatically obtained by a specific reader, which was based on silver amplification immunochromatography system, in 15 min. The detection of AdV was confirmed by real-time polymerase chain reaction (PCR) method, and typing was performed by direct sequencing. Comparative dilution assay was carried out with the two kits, using AdV type 3 and type 54 strains.

Results: The sensitivity of the control kit and test kit was 89.8% and 98.3%, respectively. The specificity of both kits was 100%. A significant difference in the sensitivities of the two IC kits against PCR positivity was observed (P < 0.01). A significant correlation was found between AdV DNA copy numbers on a logarithmic scale obtained with the two tests (P < 0.01). The sensitivity of the test kit was 32-64-fold higher than that of the control kit without silver amplification for both AdV types.

Conclusions: These results suggest that this novel amplified AdV detection kit using tears including conjunctival exudate is useful, because it decreases patients' discomfort from specimen collection and its sensitivity is significantly higher than that of the conventional IC kit.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00417-019-04281-7DOI Listing
April 2019
1 Read

Publication Analysis

Top Keywords

including conjunctival
12
tears including
12
conjunctival exudate
12
patients adenoviral
8
amplification immunochromatography
8
silver amplification
8
test kit
8
exudate patients
8
control kit
8
kit
6
test
5
kits
5
specific reader
4
reader based
4
automatically specific
4
confirmed real-time
4
kits automatically
4
based silver
4
immunochromatography system
4
collected pressing
4

References

(Supplied by CrossRef)
Article in Epidemiol Infect
L Zhang et al.
Epidemiol Infect 2016
Article in J Infect Dis
JT Grayston et al.
J Infect Dis 1956
Article in J Clin Microbiol
R Miura-Ochiai et al.
J Clin Microbiol 2007
Article in Ophthalmology
E Uchio et al.
Ophthalmology 1997
Article in J Infect Chemother
K Ouchi et al.
J Infect Chemother 1999
Article in J Virol Methods
A Wada et al.
J Virol Methods 2011
Article in J Chromatogr B Analyt Technol Biomed Life Sci
IH Cho et al.
J Chromatogr B Analyt Technol Biomed Life Sci 2010
Article in Pediatr Int
M Watanabe et al.
Pediatr Int 2005
Article in J Clin Microbiol
Z Gu et al.
J Clin Microbiol 2003
Article in Mol Biol Evol
K Tamura et al.
Mol Biol Evol 2011
Article in Invest Ophthalmol Vis Sci
DW Vastine et al.
Invest Ophthalmol Vis Sci 1977

Similar Publications