Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus.

Authors:
Roger Daniel
Roger Daniel
University of Wisconsin-Madison Medical School
United States
Elita Jauneikaite
Elita Jauneikaite
Faculty of Medicine and Institute for Life Sciences
Victoria Chalker
Victoria Chalker
Royal Veterinary College
United Kingdom

BMC Res Notes 2019 Feb 14;12(1):85. Epub 2019 Feb 14.

Section of Paediatrics, Department of Medicine, Imperial College London, London, UK.

Objective: Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from these women for timely intrapartum antibiotic administration to prevent neonatal disease. Many laboratories now use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) by direct plating or cell lysis for the identification of GBS isolates. The cell lysis step increases time to results for clinical samples and is more complex to perform. Therefore, we seek to evaluate the sensitivity and specificity of the quicker and more rapid direct plating method in identifying GBS.

Results: We directly compared swab isolates analysed by both direct plating and cell lysis method and demonstrated that direct plating has a sensitivity and specificity of 0.97 and 1, respectively, compared to an additional cell lysis step. We demonstrated that MALDI-TOF MS can be successfully used for batch processing by the direct plating method which saves time. These results are reassuring for laboratories worldwide who seek to identify GBS from swabs samples as quickly as possible.

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Source
http://dx.doi.org/10.1186/s13104-019-4119-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376729PMC
February 2019
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