Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.

Authors:
Kanya C Long
Kanya C Long
University of Texas Medical Branch
United States
Juan Sulca
Juan Sulca
Centro de Investigación de Enfermedades Tropicales
Isabel Bazan
Isabel Bazan
New York University School of Medicine and Bellevue Hospital Center
United States
Helvio Astete
Helvio Astete
University S. Naval Medical Research Unit No.6 (NAMRU-6) Iquitos Laboratory
Stalin Vilcarromero
Stalin Vilcarromero
University of California
United States

PLoS Negl Trop Dis 2019 02 12;13(2):e0007116. Epub 2019 Feb 12.

Department of Entomology and Nematology, University of California, Davis, Davis, California, United States of America.

Background: Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru.

Methodology/principal Findings: Our study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. Mosquitoes were tested after approximately 14 days of extrinsic incubation for DENV infection and dissemination. A total of 58 participants, with viremias ranging from 1.3 × 10(2) to 2.9 × 10(6) focus-forming units per mL of serum, participated in one or more feeding methods. DENV infection and dissemination rates were not significantly different following direct and indirect-EDTA feeding; however, they were significantly lower for mosquitoes that fed indirectly on blood with no additive. Relative to direct feeding, infection rates showed greater variation following indirect-EDTA than indirect-no additive feeding. Dissemination rates were similar across all feeding methods. No differences were detected in DENV infection or dissemination rates in mosquitoes fed directly on participants with different dengue illness severity.

Conclusions/significance: Our study demonstrates the feasibility of using direct and indirect feeding methods for field-based studies on vector competence. Direct mosquito feeding is preferable in terms of logistical ease, biosecurity, and reliability.

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Source
http://dx.doi.org/10.1371/journal.pntd.0007116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388938PMC
February 2019
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