Serological evidence of infection with dengue and Zika viruses in horses on French Pacific Islands.

Authors:
Isabelle Leparc-Goffart
Isabelle Leparc-Goffart
Karolinska Institutet
Sweden
Denise Desoutter
Denise Desoutter
Veterinary Diagnostic Laboratory
Steeve Lowenski
Steeve Lowenski
Université Paris Est Créteil (UPEC)
France
Marine Dumarest
Marine Dumarest
Laboratory of Pathogen Discovery
Gaelle Gonzalez
Gaelle Gonzalez
Medical Research Council-University of Glasgow Centre for Virus Research

PLoS Negl Trop Dis 2019 02 7;13(2):e0007162. Epub 2019 Feb 7.

UMR 1161 Virology, ANSES, INRA, ENVA, ANSES Animal Health Laboratory, EURL for equine diseases, Maisons-Alfort, France.

New Caledonia and French Polynesia are areas in which arboviruses circulate extensively. A large serological survey among horses from New Caledonia and French Polynesia was carried out to investigate the seroprevalence of flaviviruses in the horse population. Here, 293 equine sera samples were screened for flaviviruses using a competitive enzyme-linked immunosorbent assay (cELISA). The positive sera were then confirmed using a flavivirus-specific microsphere immunoassay (MIA) and seroneutralization tests. This serosurvey showed that 16.6% (27/163) and 30.8% (40/130) of horses were positive for cELISA tests in New Caledonia and French Polynesia, respectively, but the MIA technique, targeting only flaviviruses causing neuro-invasive infections in humans and horses (i.e. West Nile virus [WNV], Japanese encephalitis virus [JEV] and tick-borne encephalitis virus [TBEV]), showed negative results for more than 85% (57/67) of the cELISA-positive animals. Seroneutralization tests with the main flaviviruses circulating in the South Pacific revealed that 6.1% (10/163; confidence interval [95% CI] 3.0%-11.0%) of sera in New Caledonia and 7.7% (10/130; 95% CI 3.8%-13.7%) in French Polynesia were positive for dengue virus serotype 1 (DENV1) and 4.3% (7/163; 95% CI 1.7%-8.6%) in New Caledonia and 15.4% (20/130, 95% CI 9.7%-22.8%) in French Polynesia were found positive for Zika virus (ZIKV). Seroprevalence of the JEV and WNV flaviviruses on the 293 samples from both island groups were comparatively much lower (less than 2%). This seroprevalence study in the horse population shows that horses can be infected with dengue and Zika viruses and that these infections lead to seroconversions in horses. The consequences of these infections in horses and their role in ZIKV and DENV epidemiological cycles are two issues that deserve further investigation.

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pntd.0007162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382171PMC
February 2019
5 Reads

Publication Analysis

Top Keywords

french polynesia
20
caledonia french
12
zika viruses
8
seroneutralization tests
8
encephalitis virus
8
dengue zika
8
horse population
8
polynesia positive
8
horses
7
french
6
caledonia
5
polynesia
5
virus
5
flaviviruses
5
flaviviruses circulating
4
main flaviviruses
4
south pacific
4
revealed 61%
4
confidence interval
4
pacific revealed
4

References

(Supplied by CrossRef)
Zika Virus
L.R. Petersen et al.
N Engl J Med 2016
Dengue in the Pacific—an update of the current situation
N. Singh et al.
Pac Health Dialog 2005
Emerging arboviruses in the Pacific
V.M. Cao-Lormeau et al.
Lancet 2014
Dengue virus type 3, South Pacific Islands, 2013
V.M. Cao-Lormeau et al.
Emerg Infect Dis 2014
Zika virus, French polynesia, South pacific, 2013
V.M. Cao-Lormeau et al.
Emerg Infect Dis 2014
Detection of Zika virus in urine
A.C. Gourinat et al.
Emerg Infect Dis 2015

Similar Publications