Molecular determination of suspected alveolar echinococcosis requiring surgical treatment in human cases from Poland

Authors:
Monika Dybicz
Monika Dybicz
Medical University of Warsaw
Marcin Padzik
Marcin Padzik
Medical University of Warsaw
Poland
Wanda Baltaza
Wanda Baltaza
Medical University of Warsaw
Lidia Chomicz
Lidia Chomicz
University of GdaƄsk
Poland

Ann Parasitol 2018;64(4):339-342

Department of Medical Biology, Medical University of Warsaw, ul. Nowogrodzka 73, 02-018 Warsaw, Poland

In this study five cases of suspected alveolar echinococcosis from Poland in which surgical treatment was needed, previously diagnosed by means of imaging and serological techniques, were analyzed in terms to identify the causative agent. Samples of the parasite tissues taken perioperatively from the liver lesions were used for the histopathological and molecular examinations. The sequences of all isolates were identical to Echinococcus multilocularis; all nad1 sequences have been deposited in GenBank The histopathological examination revealed Passpositive fragments of laminated layers typical for E. multilocularis metacestode. Obtained results confirm that the use of imaging techniques only may be insufficient to diagnose alveococcosis thus the recognition of the zoonosis should base on several procedures; especially valuable are highly sensitive and specific molecular methods.

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April 2019
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