Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.

Nat Commun 2019 01 30;10(1):500. Epub 2019 Jan 30.

School of Biosciences, University of Nottingham, Sutton Bonington Campus, Nottingham, LE12 5RD, UK.

High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blastocysts. We show that following an emergent short naive pluripotent signature in early embryos, there is a protracted appearance of a primed signature in advanced embryonic stages. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-019-08387-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353908PMC
January 2019
10.742 Impact Factor

Publication Analysis

Top Keywords

single cell
8
embryos single
8
embryos
5
early
5
epiblast hypoblast
4
blastocysts emergent
4
late blastocysts
4
hypoblast late
4
emergent short
4
short naive
4
embryos protracted
4
protracted appearance
4
appearance primed
4
early embryos
4
signature early
4
naive pluripotent
4
pluripotent signature
4
blastocysts epiblast
4
mass trophectoderm
4
delineation analysed
4

Similar Publications