Evaluation of cross-reactivity to Taenia hydatigena and Echinococcus granulosus in the enzyme-linked immunoelectrotransfer blot assay for the diagnosis of porcine cysticercosis.

Authors:
Yesenia Castillo
Yesenia Castillo
School of Sciences
Peru
Maria Teresa Lopez-Urbina
Maria Teresa Lopez-Urbina
School of Veterinary Medicine
Peru
Pierre Dorny
Pierre Dorny
Institute of Tropical Medicine
Antwerpen | Belgium
Hector H Garcia
Hector H Garcia
School of Sciences
Peru
Armando E Gonzalez
Armando E Gonzalez
School of Veterinary Medicine
Peru

Parasit Vectors 2019 Jan 24;12(1):57. Epub 2019 Jan 24.

Center for Global Health Tumbes, Universidad Peruana Cayetano Heredia, Tumbes, Peru.

Background: Taenia solium is an important zoonotic parasite that infects humans as definitive host (taeniasis) and pigs as intermediate host (cysticercosis). Serological diagnosis of porcine cysticercosis is limited to antigen detection using ELISA, which is known to cross-react with other Taenia species, and antibody detection using the lentil-lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB), which has not been adequately evaluated for cross-reactivity to other parasites. Field studies suggest that the GP50 diagnostic band of the LLGP EITB may cross-react to Taenia hydatigena, a common non-zoonotic parasitic infection of pigs. The objective of this study was to evaluate the specificity of the LLGP EITB assay in pigs infected experimentally with T. hydatigena and Echinococcus granulosus.

Results: Twelve three-month-old seronegative were divided into two groups; six were each given an oral challenge with a single gravid proglottid of T. hydatigena and the other six were each given an oral challenge with 50 gravid proglottids of E. granulosus. Serum samples were collected biweekly until 14 weeks when all pigs underwent a detailed necropsy. Taenia hydatigena cysticerci were found in two of six pigs from the first group. Four T. hydatigena-exposed pigs were seropositive at the GP50-band only on EITB LLGP; two of these had cysts at necropsy while no seronegative pigs had cysts. One E. granulosus-exposed pig was positive to EITB LLGP, again with reactivity only to GP50; all six pigs had hepatic echinococcosis on necropsy.

Conclusion: These results provide definitive evidence that the GP50 diagnostic band in pigs cross-reacts with T. hydatigena. Evidence of cross-reaction with E. granulosus was not conclusive.

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Source
https://parasitesandvectors.biomedcentral.com/articles/10.11
Publisher Site
http://dx.doi.org/10.1186/s13071-018-3279-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346519PMC
January 2019
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References

(Supplied by CrossRef)
Article in PLoS Negl Trop Dis.
PC Ndimubanzi et al.
PLoS Negl Trop Dis. 2010
Article in Am J Trop Med Hyg.
AE Gonzalez et al.
Am J Trop Med Hyg. 1994
Article in Am J Trop Med Hyg.
HH Garcia et al.
Am J Trop Med Hyg. 2003
Article in PLoS Negl Trop Dis.
SE O’Neal et al.
PLoS Negl Trop Dis. 2014
Article in PLoS Negl Trop Dis.
CM Gavidia et al.
PLoS Negl Trop Dis. 2013
Article in Trans R Soc Trop Med Hyg.
VR Mohan et al.
Trans R Soc Trop Med Hyg. 2013
Article in Vet J.
CS Sikasunge et al.
Vet J. 2008
Article in J Infect Dis.
VCW Tsang et al.
J Infect Dis. 1989
Article in Vet Immunol Immunopathol.
VCW Tsang et al.
Vet Immunol Immunopathol. 1991
Article in Vet Parasitol.
P Dorny et al.
Vet Parasitol. 2017
Article in N Engl J Med.
HH Garcia et al.
N Engl J Med. 2016

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