Thiopurine intolerance-causing mutations in NUDT15 induce temperature-dependent destabilization of the catalytic site.

Authors:
Petr Man, PhD
Petr Man, PhD
Institute of Microbiology
Prague | Czech Republic
Daniel Kavan
Daniel Kavan
Institute of Microbiology
Czech Republic
Petr Novak
Petr Novak
Institute of Plant Molecular Biology
New Delhi | India

Biochim Biophys Acta Proteins Proteom 2019 Apr 10;1867(4):376-381. Epub 2019 Jan 10.

Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nam. 2, Prague 6 166 10, Czech Republic. Electronic address:

Germline mutations in NUDT15 cause thiopurine intolerance during treatment of leukemia or autoimmune diseases. Previously, it has been shown that the mutations affect the enzymatic activity of the NUDT15 hydrolase due to decreased protein stability in vivo. Here we provide structural insights into protein destabilization in R139C and V18I mutants using thermolysin-based proteolysis and H/D exchange followed by mass spectrometry. Both mutants exhibited destabilization of the catalytic site, which was more pronounced at higher temperature. This structural perturbation is shared by the mutations despite their different positions within the protein structure. Reaction products of NUDT15 reverted these conformational abnormalities, demonstrating the importance of ligands for stabilization of a native state of the mutants. This study shows the action of pharmacogenetic variants in NUDT15 in a context of protein structure, which might open novel directions in personalized chemotherapy.

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http://dx.doi.org/10.1016/j.bbapap.2019.01.006DOI Listing
April 2019
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