Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue (TBO) inhibits both growth and dimorphism in Paracoccidioides brasiliensis by a mechanism involving reactive oxygen species (ROS) production.

Authors:
Flavia Villaca Morais
Flavia Villaca Morais
Laboratory of Cellular and Molecular Biology of Fungi
Maricilia Silva Costa
Maricilia Silva Costa
Instituto de Pesquisa & Desenvolvimento

Photodiagnosis Photodyn Ther 2019 Mar 3;25:421-424. Epub 2019 Jan 3.

Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba - UNIVAP, Av. Shishima Hifumi, 2911, São José dos Campos, SP, Brazil. Electronic address:

Background: The thermo-dimorphic fungus Paracoccidioides brasiliensis is the pathogen of Paracoccidioidomycosis, an important public health problem in Latin American with prevalence in Brazil. Photodynamic Antimicrobial Chemotherapy (PACT) is a process that combines a photosensitizer and light, producing reactive oxygen species (ROS) that can promote damages to treated cells.

Methods: In this work was study the effect of PACT, using Toluidine blue (TBO) on both yeast and mycelial cells of P. brasiliensis.

Results: It was observed that PACT decreased P. brasiliensis yeast growth, in a dependent manner of both TBO concentrations and fluence. In the presence of TBO 0.005 mg/mL, PACT reduced P. brasiliensis yeast growth in 63, 62 and 86%, using fluences of 20, 30 and 40 J/cm, respectively. After PACT, ROS production increased 2.80, 4.64 and 7.90 times, in the presence of TBO 0.001, 0.002 and 0.005 mg/mL, respectively. It was observed that after PACT, the cells are predominantly in mycelia form, indicating that mycelial cells irradiated in the presence of TBO, maintained their filamentous form and absence and/or decreased presence of transition structures.

Conclusions: These results demonstrated the potential of PACT, using TBO to inhibit both yeast and mycelium development of P. brasiliensis.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S15721000183037
Publisher Site
http://dx.doi.org/10.1016/j.pdpdt.2019.01.003DOI Listing

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March 2019
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