Comparative proteomic analysis of salt-responsive proteins in canola roots by 2-DE and MALDI-TOF MS.

Authors:
Mahmoud Toorchi
Mahmoud Toorchi
National Institute of Crop Science
Japan
Ali Bandehagh
Ali Bandehagh
Tabriz University of Medical Sciences
Iran
Anna Ostendorp
Anna Ostendorp
University of Oldenburg
Germany
Patrizia Hanhart
Patrizia Hanhart
University of Osnabrück
Julia Kehr
Julia Kehr
Max-Planck-Institute of Molecular Plant Physiology
Germany

Biochim Biophys Acta Proteins Proteom 2019 Mar 3;1867(3):227-236. Epub 2019 Jan 3.

Molecular Plant Genetics, Universität Hamburg, Biozentrum Klein Flottbek, Ohnhorststr. 18, 22609 Hamburg, Germany. Electronic address:

Salinity stress is a major abiotic stress that affects plant growth and limits crop production. Roots are the primary site of salinity perception, and salt sensitivity in roots limits the productivity of the entire plant. To better understand salt stress responses in canola, we performed a comparative proteomic analysis of roots from the salt-tolerant genotype Safi-7 and the salt-sensitive genotype Zafar. Plants were exposed to 0, 150, and 300 mM NaCl. Our physiological and morphological observations confirmed that Safi-7 was more salt-tolerant than Zafar. The root proteins were separated by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry was applied to identify proteins regulated in response to salt stress. We identified 36 and 25 protein spots whose abundance was significantly affected by salt stress in roots of plants from the tolerant and susceptible genotype, respectively. Functional classification analysis revealed that the differentially expressed proteins from the tolerant genotype could be assigned to 14 functional categories, while those from the susceptible genotype could be classified into 9 functional categories. The most significant differences concerned proteins involved in glycolysis (Glyceraldehyde-3-phosphate dehydrogenase, Fructose-bisphosphate aldolase, Phosphoglycerate kinase 3), stress (heat shock proteins), Redox regulation (Glutathione S-transferase DHAR1, L-ascorbate peroxidase), energy metabolism (ATP synthase subunit B), and transport (V-type proton ATPase subunit B1) which were increased only in the tolerant line under salt stress. Our results provide the basis for further elucidating the molecular mechanisms of salt-tolerance and will be helpful for breeding salt-tolerant canola cultivars.

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Source
http://dx.doi.org/10.1016/j.bbapap.2018.12.009DOI Listing
March 2019

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