Helminth infections of wild European gray wolves (Canis lupus Linnaeus, 1758) in Lower Saxony, Germany, and comparison to captive wolves.

Authors:
Andrea Springer
Andrea Springer
Universitätsklinikum
Germany
Christina Strube
Christina Strube
Institute for Parasitology

Parasitol Res 2019 Feb 4;118(2):701-706. Epub 2019 Jan 4.

Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Bünteweg 17, 30159, Hannover, Germany.

This study aimed to investigate the endoparasite fauna of wild European gray wolves, which are currently recolonizing Germany. In total, 69 fecal samples of wild wolves were collected in Lower Saxony, Germany, from 2013 to 2015, analyzed by the sedimentation-flotation and McMaster techniques and compared to previous results on captive European Gray wolves living in zoological gardens in Germany. In addition to coproscopy, taeniid-positive samples from wild as well as captive wolves were differentiated by amplification and sequencing of small subunit ribosomal RNA (SSU rRNA) and NADH dehydrogenase 1 (nad1) gene fragments. Missing Taenia krabbei SSU rRNA reference sequences were generated from two T. krabbei specimens. Overall, 60.87% (42/69) of wild wolve samples were microscopically positive for at least one of seven egg types. Capillaria/Eucoleus spp. showed the highest frequency (31.88% [22/69]), followed by Taeniidae (21.74% [15/69]), Ancylostomatidae (20.29% [14/69]), Alaria alata (15.94% [11/69]), Toxocara canis (13.04% [9/69]), and Toxascaris leonina and Trichuris vulpis (each 5.80% [4/69]). Amplification of SSU rRNA was successful for 7/15 Taeniidae-positive samples from wild and 20/39 samples from captive wolves, revealing T. hydatigena in two and 14 samples, respectively. Taenia krabbei was detected in two further samples of wild and three samples of captive wolves, while for the remaining samples, no differentiation between T. serialis/T. krabbei was possible. Echinococcus spp. were not detected. Sequence comparisons revealed that the SSU rRNA gene fragment was not suitable to differentiate between T. serialis and T. krabbei. Therefore, the use of this fragment alone cannot be recommended for species identification in future studies.

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http://dx.doi.org/10.1007/s00436-018-6181-3DOI Listing
February 2019
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References

(Supplied by CrossRef)
Article in Parasitol Int
MT Armua-Fernandez et al.
Parasitol Int 2011
Article in Parasitol Res
AC Becker et al.
Parasitol Res 2016
Article in Parasitology
HM Bryan et al.
Parasitology 2012
Article in Int J Parasitol Parasites Wildl
D Guerra et al.
Int J Parasitol Parasites Wildl 2013
Article in Vet Parasitol
C Hermosilla et al.
Vet Parasitol 2017
Article in Sci Rep
I Lesniak et al.
Sci Rep 2017
Article in Ecol Evol
I Lesniak et al.
Ecol Evol 2018
Article in Northern Italian Apennines. Int J Parasitol Parasites Wildl
G Poglayen et al.
Northern Italian Apennines. Int J Parasitol Parasites Wildl 2017
Article in Dtsch Tierarztl Wochenschr
PN Prelezov et al.
Dtsch Tierarztl Wochenschr 2003
Article in Parasitol Res
K Riehn et al.
Parasitol Res 2012

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