iTRAQ-based phosphoproteomic analysis reveals host cell's specific responses to Toxoplasma gondii at the phases of invasion and prior to egress.

Authors:
Cheng He
Cheng He
Second Military Medical University
China
Ling Kong
Ling Kong
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine
Yueyang Shi | China
Santhosh Puthiyakunnon
Santhosh Puthiyakunnon
Southern Medical University
China
Hai-Xia Wei
Hai-Xia Wei
Heilongjiang University
China
Li-juan Zhou
Li-juan Zhou
Institute of Chemistry
China
Hong-Juan Peng
Hong-Juan Peng
Southern Medical University
China

Biochim Biophys Acta Proteins Proteom 2019 Mar 18;1867(3):202-212. Epub 2018 Dec 18.

Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China. Electronic address:

Protein phosphorylation plays a key role in host cell-T. gondii interaction. However, the phosphoproteome data of host cell at various phases of T. gondii infection has not been thoroughly described. In this study, we assessed the host phosphoproteome data with isobaric tags for relative and absolute quantification (iTRAQ) method during the phases of T. gondii invasion (30 min post infection, PI) and prior to egress (28 h PI). Our iTRAQ analysis revealed a total of 665 phosphoproteins, among which the significantly regulated phosphoproteins in different between-group comparisons were further analyzed. Functional analysis of these significantly regulated phosphoproteins suggested that T. gondii modulated host cell processes through phosphorylation including cell cycle regulation, inducing apoptosis, blocking the synthesis of some inflammatory factors, mediating metabolism to support its proliferation at the infection phase prior to egress, and utilizing membrane and energy from host cell, reorganizing cytoskeleton to favor its invasion and PV formation at the phase of invasion. The phosphorylation level of Smad2, CTNNA1, and HSPB1 identified with western blot revealed a consistent trend of change with iTRAQ result. These newly identified and significantly regulated phosphoproteins from our phosphoproteome data may provide new clues to unravel the host cell's complex reaction against T. gondii infection and the interaction between the host cell and T. gondii.

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Source
http://dx.doi.org/10.1016/j.bbapap.2018.12.004DOI Listing
March 2019

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