Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development.

PLoS One 2018 17;13(12):e0208851. Epub 2018 Dec 17.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

In response to the aggressive global spread of the mosquito-borne chikungunya virus (CHIKV), an accurate and accessible diagnostic tool is of high importance. CHIKV, an arthritogenic alphavirus, comprises three genotypes: East/Central/South African (ECSA), West African (WA), and Asian. A previous rapid immunochromatographic (IC) test detecting CHIKV E1 protein showed promising performance for detection of the ECSA genotype. Unfortunately, this kit exhibited lower capacity for detection of the Asian genotype, currently in circulation in the Americas, reflecting the low avidity of one of the monoclonal antibodies (mAbs) in this IC kit for the E1 protein of the Asian-genotype because of a variant amino acid sequence. To address this shortcoming, we set out to generate a new panel of broad-spectrum mouse anti-CHIKV mAbs using hybridoma technology. We report here the successful generation of mouse anti-CHIKV mAbs targeting CHIKV E1 and capsid proteins. These mAbs possessed broad reactivity to all three CHIKV genotypes, while most of the mAbs lacked cross-reactivity towards Sindbis, dengue, and Zika viruses. Two of the mAbs also lacked cross-reactivity towards other alphaviruses, including O'nyong-nyong, Ross River, Mayaro, Western Equine Encephalitis, Eastern Equine Encephalitis, and Venezuelan Equine Encephalitis viruses. In addition, another two mAbs cross-reacted weakly only with most closely related O'nyong-nyong virus. Effective diagnosis is one of the keys to disease control but to date, no antibody-based rapid IC platform for CHIKV is commercially available. Thus, the application of the mAbs characterized here in the rapid diagnostic IC kit for CHIKV detection is expected to be of great value for clinical diagnosis and surveillance purposes.

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208851PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296674PMC

Still can't find the full text of the article?

We can help you send a request to the authors directly.
May 2019
15 Reads

Publication Analysis

Top Keywords

equine encephalitis
12
lacked cross-reactivity
8
chikungunya virus
8
mabs lacked
8
rapid diagnostic
8
antibody-based rapid
8
mabs
8
mouse anti-chikv
8
anti-chikv mabs
8
monoclonal antibodies
8
chikv
7
virus effective
4
effective diagnosis
4
kit protein
4
protein asian-genotype
4
asian-genotype variant
4
variant amino
4
sequence address
4
weakly closely
4
set generate
4

References

(Supplied by CrossRef)
An outbreak of chikungunya fever in the province of Ravenna, Italy.
R Angelini et al.
Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 2007
Global distribution and environmental suitability for chikungunya virus, 1952 to 2015
EO Nsoesie et al.
Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 2016
Understanding the evolution and spread of chikungunya virus in the Americas using complete genome sequences
NSD Sahadeo et al.
Virus Evol 2017
Transcontinental movement of Asian genotype chikungunya virus
RS Lanciotti et al.
Emerging infectious diseases 2014
Assessing the origin of and potential for international spread of chikungunya virus from the Caribbean
K Khan et al.
PLoS Curr 2014
Therapeutics and vaccines against chikungunya virus
T Ahola et al.
Vector borne and zoonotic diseases 2015

Similar Publications