yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs.

Authors:
Stefan Frank
Stefan Frank
Klinikum der Johann Wolfgang Goethe-Universität
Germany
Gaurav Ahuja
Gaurav Ahuja
Universität zu Köln
Germany
Deniz Bartsch
Deniz Bartsch
Max Planck Institute for Metabolism Research
Wenjie Yao
Wenjie Yao
Peking University
Vijay Suresh Akhade
Vijay Suresh Akhade
a Molecular Biology and Genetics Unit; Jawaharlal Nehru Center for Advanced Scientific Research; Jakkur P. O. ; Bangalore
India

Cell Stem Cell 2019 Feb 13;24(2):318-327.e8. Epub 2018 Dec 13.

Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany; Institute for Neurophysiology, University of Cologne, 50931 Cologne, Germany; CECAD, Cologne Cluster of Excellence in Cellular Stress Responses in Ageing-Associated Diseases, University of Cologne, 50931 Cologne, Germany. Electronic address:

Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.

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http://dx.doi.org/10.1016/j.stem.2018.11.005DOI Listing
February 2019
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