Effects of Moringa oleifera Lam Extract (MOLE) in the heat shock protein 70 expression and germ cell apoptosis on experimentally induced cryptorchid testes of rats.

Authors:
Muhammetnur Tekayev
Muhammetnur Tekayev
Department of Histology and Embryology, Faculty of Medicine, Institute of Health Sciences, University of Gaziantep
Gaziantep 27310, Turkey
Embryology
Gaziantep, Şehitkamil | Turkey
Nuray Bostancieri
Nuray Bostancieri
Institute of Health Sciences
Mehmet Turker
Mehmet Turker
Kirikkale University School of Medicine
Turkey
Hulya Cicek
Hulya Cicek
Gaziantep University Hospital
Gaziantep | Turkey
Kaifee Arman, PhD
Kaifee Arman, PhD
Gaziantep University
Gaziantep | Turkey

Gene 2019 Mar 6;688:140-150. Epub 2018 Dec 6.

Institut de recherches cliniques de Montreal (IRCM), Montreal, QC, Canada; Division of Experimental Medicine, McGill University, Montreal, QC, Canada.

Moringa oleifera (Moringaceae) is a plant known for having high antioxidant potency, anticancer, hepatoprotective, cardioprotective etc. and many more activities. Besides these, Moringaceae has the potential for attenuating the male sexual dysfunction. Reactive oxygen species/ROS were increased in cryptorchidism and therefore cause infertility by damaging sperm DNA and germ cell apoptosis. There was an increase in heat shock proteins (HSP) in cells, which is affected by heat shock. In the present study, the antioxidant effects of two different doses of M. oleifera Lam Extract (MOLE) on experimentally induced cryptorchid testes of rats was investigated. Forty two male rats (16?days old) were divided into four groups: a normal control group, a cryptorchidism-induced control group and two cryptorchidism-induced groups treated orally with either 400 or 800?mg/kg MOLE for 2?weeks. Our study showed that there were ruptures from interstitial spaces, separation of the germ cells from basal membrane, falling of the germ cells into the lumen, perivascular fibrosis, oedema, increased level of HSP70, apoptosis, malondialdehyde (MDA) and decrease in the level of superoxide dismutase (SOD) after the cryptorchidism. We found that pathological damages, oxidative stress, expression of the HSP70 and germ cell apoptosis were decreased in treated groups with MOLE. In brief, we can say that aqueous extract of M. oleifera reduces the oxidative stress in a unilateral cryptorchidism induced rats, and it might attenuate histopathological damages, HSP expression and germ cell apoptosis.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S03781119183124
Publisher Site
http://dx.doi.org/10.1016/j.gene.2018.11.091DOI Listing
March 2019
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2.498 Impact Factor

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