Female Sex Development and Reproductive Duct Formation Depend on Wnt4a in Zebrafish.

Genetics 2019 01 16;211(1):219-233. Epub 2018 Nov 16.

Department of Molecular and Cellular Biology, University of California, Davis, California 95616

In laboratory strains of zebrafish, sex determination occurs in the absence of a typical sex chromosome and it is not known what regulates the proportion of animals that develop as males or females. Many sex determination and gonad differentiation genes that act downstream of a sex chromosome are well conserved among vertebrates, but studies that test their contribution to this process have mostly been limited to mammalian models. In mammals, WNT4 is a signaling ligand that is essential for ovary and Müllerian duct development, where it antagonizes the male-promoting FGF9 signal. Wnt4 is well conserved across all vertebrates, but it is not known if Wnt4 plays a role in sex determination and/or the differentiation of sex organs in nonmammalian vertebrates. This question is especially interesting in teleosts, such as zebrafish, because they lack an Fgf9 ortholog. Here we show that is the ortholog of mammalian , and that was present in the last common ancestor of humans and zebrafish, but was lost in mammals. We show that loss-of-function mutants develop predominantly as males and conclude that activity promotes female sex determination and/or differentiation in zebrafish. Additionally, both male and female mutants are sterile due to defects in reproductive duct development. Together these results strongly argue that Wnt4a is a conserved regulator of female sex determination and reproductive duct development in mammalian and nonmammalian vertebrates.

Download full-text PDF

Source
http://www.genetics.org/lookup/doi/10.1534/genetics.118.3016
Publisher Site
http://dx.doi.org/10.1534/genetics.118.301620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325708PMC
January 2019
4 Reads

Publication Analysis

Top Keywords

sex determination
20
reproductive duct
12
duct development
12
female sex
12
well conserved
8
develop males
8
and/or differentiation
8
conserved vertebrates
8
determination and/or
8
sex chromosome
8
nonmammalian vertebrates
8
sex
8
determination
5
zebrafish
5
role sex
4
wnt4 plays
4
plays role
4
differentiation sex
4
question interesting
4
interesting teleosts
4

References

(Supplied by CrossRef)

Similar Publications