Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae.

Authors:
Weifeng Zhu
Weifeng Zhu
Nanchang University
China
Chao Wu
Chao Wu
Nanjing Drum Tower Hospital
Nanjing Shi | China
Chao Kang
Chao Kang
Hunan University
China
Chengzhi Cai
Chengzhi Cai
University of Houston
United States
Ya Wang
Ya Wang
Neuropsychology and Applied Cognitive Neuroscience Laboratory
China
Jingtao Li
Jingtao Li
Sichuan University
China
Qiang Zhang
Qiang Zhang
Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology
China
Xiaomei Sun
Xiaomei Sun
Northwest A&F University
China

Vaccine 2018 12 13;36(52):8079-8083. Epub 2018 Nov 13.

Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. Electronic address:

Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Bacterial surface proteins are promising vaccine candidates. We recently identified 3 E. rhusiopathiae surface proteins (GAPDH, HP0728, and HP1472) and characterized their roles as virulence factors. However, their efficacy as protective antigens is still unknown. The N-terminal region of a previously identified surface protein, CbpB (CbpB-N), is speculated to be a protective antigen, but this needs to be verified. The aim of this study was to evaluate the protective efficacy of GAPDH, HP0728, HP1472, and CbpB-N. Immunization with recombinant GAPDH provided complete protection in a mouse model, recombinant CbpB-N provided partial protection, while recombinant HP0728 and HP1472 provided no protection. Recombinant GAPDH also provided good protection in a pig model. GAPDH antiserum exhibited significant blood bactericidal activity against E. rhusiopathiae. In conclusion, GAPDH and CbpB-N were found to be protective antigens of E. rhusiopathiae, and GAPDH is a promising vaccine candidate.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S0264410X183145
Publisher Site
http://dx.doi.org/10.1016/j.vaccine.2018.10.071DOI Listing

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December 2018
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