Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections.

Authors:
Vikas Taank
Vikas Taank
Dept of Biological Sciences, Old Dominion University
Doctoral Candidate
Norfolk, VA | United States
Wenshuo Zhou
Wenshuo Zhou
Old Dominion University
Xuran Zhuang
Xuran Zhuang
Shanghai Jiao Tong University School of Medicine
China
John F Anderson
John F Anderson
Yale University School of Medicine
United States
Utpal Pal
Utpal Pal
University of Maryland
College Park | United States
Hameeda Sultana
Hameeda Sultana
Yale University School of Medicine
New Haven | United States
Girish Neelakanta
Girish Neelakanta
Yale University School of Medicine
United States

Parasit Vectors 2018 Nov 14;11(1):593. Epub 2018 Nov 14.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA.

Background: Ixodes scapularis organic anion transporting polypeptides (OATPs) play important roles in tick-rickettsial pathogen interactions. In this report, we characterized the role of these conserved molecules in ticks infected with either Lyme disease agent Borrelia burgdorferi or tick-borne Langat virus (LGTV), a pathogen closely related to tick-borne encephalitis virus (TBEV).

Results: Quantitative real-time polymerase chain reaction analysis revealed no significant changes in oatps gene expression upon infection with B. burgdorferi in unfed ticks. Synchronous infection of unfed nymphal ticks with LGTV in vitro revealed no significant changes in oatps gene expression. However, expression of specific oatps was significantly downregulated upon LGTV infection of tick cells in vitro. Treatment of tick cells with OATP inhibitor significantly reduced LGTV loads, kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), levels and expression of several oatps in tick cells. Furthermore, bioinformatics characterization of OATPs from some of the medically important vectors including ticks, mosquitoes and lice revealed the presence of several glycosylation, phosphorylation and myristoylation sites.

Conclusions: This study provides additional evidence on the role of arthropod OATPs in vector-intracellular pathogen interactions.

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Source
http://dx.doi.org/10.1186/s13071-018-3160-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236954PMC

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November 2018
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