Insulin Modulates -Induced Inflammation by Restoring the Populations of NK Cells, Dendritic Cells, and B Lymphocytes in Lungs.

J Diabetes Res 2018 22;2018:6209694. Epub 2018 Oct 22.

Laboratory of Immunoendocrinology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences of University Sao Paulo (FCF/USP), São Paulo, Brazil.

Paracoccidioidomycosis, a key issue for Brazilian health service, can be aggravated in patients with impaired immunological responses, such as diabetic patients. We evaluated the role of insulin in inflammatory parameters in diabetic and nondiabetic mice using a systemic mycosis (Pb) model. Diabetic C57BL-6 mice and controls were infected with Pb18 and treated with insulin for 12 days prior to experiments. After 55 days, infected diabetic mice exhibited fewer leukocytes in both peritoneal lavage fluid (PeLF) and bronchoalveolar lavage fluid and reduced secretion of interleukin- (IL-) 6 in lungs. In addition, diabetic mice presented a reduced influx of TCD4+ cells, TCD8+ cells, B lymphocytes, NK cells, and dendritic cells compared to control infected groups. Insulin treatment restored the leukocyte number in PeLF and restored the presence of B lymphocytes, dendritic cells, and NK cells in lungs of diabetic animals. The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection.

Download full-text PDF

Source
http://dx.doi.org/10.1155/2018/6209694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217756PMC
March 2019
20 Reads

Publication Analysis

Top Keywords

dendritic cells
16
diabetic mice
16
cells lymphocytes
12
cells
9
insulin modulates
8
restoring populations
8
lungs diabetic
8
lymphocytes lungs
8
diabetic
8
lavage fluid
8
cells dendritic
8
impaired immunological
8
mice
6
insulin
5
presence lymphocytes
4
exhibited fewer
4
lymphocytes dendritic
4
cells cells
4
mice exhibited
4
fewer leukocytes
4

References

(Supplied by CrossRef)

Similar Publications