Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium.

Cell Rep 2018 10;25(4):825-832.e5

Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, L8S 4L8, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, L8S 4L8, Canada. Electronic address:

Bacterial two-component regulatory systems (TCS) couple the detection of niche-specific cues with adaptive gene expression to optimize fitness. In Salmonella Typhimurium (STM), the SsrA-SsrB TCS regulates virulence genes needed for survival within host cells, yet the impact of this TCS on regulatory evolution in this pathogen remains incompletely understood. Here, we show that SsrB alters a transcriptional network controlling bacterial motility to limit inflammasome activation during host cell infection. Using comparative RNA sequencing between STM and S. bongori (SBG) engineered to express SsrB, we show that SsrB represses flagellar gene expression in STM but activates this pathway in SBG, which has evolved in the absence of SsrB. Motility repression in STM is driven by an SsrB-binding region upstream of flhDC that appears to have evolved in STM following divergence from SBG. These data reveal a divergent regulatory circuit in non-coding DNA that reduces flagellar gene expression to evade host defenses.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S22111247183153
Publisher Site
http://dx.doi.org/10.1016/j.celrep.2018.09.078DOI Listing
October 2018
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