Background: Patient safety cannot be considered in isolation when organizational factors, both active and latent, influence patient outcomes.Methods: We did a cross-sectional mixed methods study using a convergent parallel design at a tertiary care public sector hospital in Hyderabad, Telangana (i) to qualitatively investigate the nature and determinants of patient safety incidents occurring in the hospital; (ii) to quantify the perception of hospital staff regarding factors affecting patient safety from an organizational perspective; and (iii) to triangulate the results to highlight areas in need of improvement.Results: The most common factors affecting patient safety were situational factors, working conditions and latent organizational factors including communication systems.