A Single-Scan Inhomogeneity-Tolerant NMR Method for High-Resolution Two-Dimensional J-Resolved Spectroscopy.

IEEE Trans Biomed Eng 2019 06 12;66(6):1559-1566. Epub 2018 Oct 12.

Objective: A robust and general single-scan NMR method, SGEN-J, is proposed for real-time recording high-resolution two-dimensional (2D) homonuclear J-resolved spectra under inhomogeneous magnetic fields.

Methods: This proposed NMR method is designed based on the combination of a selective gradient encoding module to encode chemical shifts with spatial positions, and a J-modulation decoding module to reveal encoded structural information. Multi-band SGEN-J is further implemented to effectively enhance spectral sensitivity with a sustained tolerance of field inhomogeneity.

Results: The SGEN-J provides an effective way to rapidly recover chemical shifts, J coupling constants, and multiplet patterns under inhomogeneous magnetic fields. Experiments on various chemical solutions were performed to demonstrate the feasibility and effectiveness of SGEN-J. Experiments on pig marrow tissues were performed to further investigate the applicability of SGEN-J to biological samples with intrinsic susceptibility variations.

Conclusion: Based on intrinsic advantages, SGEN-J serves as a helpful complement to existing 2D J-resolved methodologies in molecular structure elucidation and biomedical study, and offer bright perspectives for real-time analyzing in vivo biological systems and monitoring in situ chemical reactions.

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http://dx.doi.org/10.1109/TBME.2018.2875797DOI Listing
June 2019
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