Functional and taxonomic classification of a greenhouse water drain metagenome.

Stand Genomic Sci 2018 5;13:20. Epub 2018 Oct 5.

1Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, 62210 Morelos Mexico.

Microbiome sequencing has become the standard procedure in the study of new ecological and human-constructed niches. To our knowledge, this is the first report of a metagenome from the water of a greenhouse drain. We found that the greenhouse is not a diverse niche, mainly dominated by and Rodobacterales. The analysis of the functions encoded in the metagenome showed enrichment of characteristic features of soil and root-associated bacteria such as ABC-transporters and hydrolase enzymes. Additionally, we found antibiotic resistances genes principally for spectinomycin, tetracycline, and aminoglycosides. This study aimed to identify the bacteria and functional gene composition of a greenhouse water drain sample and also provide a genomic resource to search novel proteins from a previously unexplored niche. All the metagenome proteins and their annotations are available to the scientific community via http://microbiomics.ibt.unam.mx/tools/metagreenhouse/.

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Source
https://standardsingenomics.biomedcentral.com/articles/10.11
Publisher Site
http://dx.doi.org/10.1186/s40793-018-0326-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173933PMC
October 2018
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