An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1.

J Alzheimers Dis 2018 ;66(2):453-460

Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX, USA.

Altered expression of α-synuclein is linked to Parkinson's disease (PD). A major challenge to explore how the increased α-synuclein affect neurotoxicity is the lack of a suitable human neuronal cell model that mimics this scenario. Its expression in neural precursors affects their differentiation process, in addition to the neuronal adaptability and variability in maintaining a constant level of expression across passages. Here, we describe an SH-SY5Y line harboring Tet-ON SNCA cDNA cassette that allows for induction of controlled α-synuclein expression after neuronal differentiation, which can be an important tool for PD research.

Download full-text PDF

Source
http://dx.doi.org/10.3233/JAD-180610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6221916PMC
October 2019
18 Reads

Publication Analysis

Top Keywords

neuronal cell
8
cell model
8
addition neuronal
4
process addition
4
differentiation process
4
neuronal adaptability
4
adaptability variability
4
maintaining constant
4
variability maintaining
4
precursors differentiation
4
neural precursors
4
model mimics
4
human neuronal
4
suitable human
4
mimics scenario
4
neurotoxicity lack
4
constant level
4
expression neural
4
lack suitable
4
expression passages
4

Similar Publications