Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.

J Mol Biol 2018 10 5;430(21):4325-4343. Epub 2018 Sep 5.

Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USA; Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA. Electronic address:

α-Neurexins are synaptic organizing molecules implicated in neuropsychiatric disorders. They bind and arrange an array of different partners in the synaptic cleft. The extracellular region of neurexin 1α (n1α) contains six LNS domains (L1-L6) interspersed by three Egf-like repeats. N1α must encode highly evolved structure-function relationships in order to fit into the narrow confines of the synaptic cleft, and also recruit its large, membrane-bound partners. Internal molecular flexibility could provide a solution; however, it is challenging to delineate because currently no structural methods permit high-resolution structure determination of large, flexible, multi-domain protein molecules. To investigate the structural plasticity of n1α, in particular the conformation of domains that carry validated binding sites for different protein partners, we used a panel of structural techniques. Individual particle electron tomography revealed that the N-terminally and C-terminally tethered domains, L1 and L6, have a surprisingly limited range of conformational freedom with respect to the linear central core containing L2 through L5. A 2.8-Å crystal structure revealed an unexpected arrangement of the L2 and L3 domains. Small-angle X-ray scattering and electron tomography indicated that incorporation of the alternative splice insert SS6 relieves the restricted conformational freedom between L5 and L6, suggesting that SS6 may work as a molecular toggle. The architecture of n1α thus encodes a combination of rigid and flexibly tethered domains that are uniquely poised to work together to promote its organizing function in the synaptic cleft, and may permit allosterically regulated and/or concerted protein partner binding.

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00222836183019
Publisher Site
http://dx.doi.org/10.1016/j.jmb.2018.08.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223652PMC
October 2018
6 Reads

Publication Analysis

Top Keywords

synaptic cleft
12
conformational freedom
8
electron tomography
8
neurexin 1α
8
structural plasticity
8
tethered domains
8
domains
5
synaptic
5
n-terminally c-terminally
4
tomography revealed
4
revealed n-terminally
4
individual particle
4
panel structural
4
structural techniques
4
techniques individual
4
particle electron
4
surprisingly limited
4
respect linear
4
linear central
4
central core
4

Similar Publications