Immunohistochemical Cross-Reactivity Between Paracoccidioides sp. from Dolphins and Histoplasma capsulatum.

Authors:
Keiichi Ueda
Keiichi Ueda
School of Veterinary Medicine
Japan
Sayaka Yamaguchi
Sayaka Yamaguchi
University of the Ryukyus
Japan
Takashi Kaneshima
Takashi Kaneshima
Kitasato University
Japan
Toshihiro Konno
Toshihiro Konno
University of Kansas Medical Center
United States
Yoshie Terashima
Yoshie Terashima
University of the Ryukyus
Japan
Atsushi Yamamoto
Atsushi Yamamoto
Toyama Institute of Health
Japan

Mycopathologia 2018 Oct 30;183(5):793-803. Epub 2018 Aug 30.

Ryukyu Animal Medical Center, Yone 5-27, Tomigusuku, Okinawa, 901-0224, Japan.

Paracoccidioidomycosis ceti is a cutaneous disease of cetaceans caused by uncultivated Paracoccidioides brasiliensis or Paracoccidioides spp. Serological cross-reactions between paracoccidioidomycosis ceti and paracoccidioidomycosis, paracoccidioidomycosis and histoplasmosis, and paracoccidioidomycosis and coccidioidomycosis have been reported before. The present study aimed to detect immunohistochemical cross-reaction between antibodies to Paracoccidioides sp. and Histoplasma capsulatum, and vice versa. Thirty murine sera, obtained from experimental infections of 6 isolates of H. capsulatum, were reacted with paraffin-embedded yeast-form cells of Paracoccidioides sp. derived from a case of paracoccidioidomycosis ceti in Japan. The murine sera were also reacted with human isolates of H. capsulatum yeast cells, with P. brasiliensis yeast cells, and with fungal cells of Coccidioides posadasii. Three dolphins' sera from cases of paracoccidioidomycosis ceti, two human sera from patients with paracoccidioidomycosis, and a serum from a healthy person with a history of coccidioidomycosis were used in order to determine that the tested fungal cells reacted properly. Sera derived from mice infected with an isolate of H. capsulatum reacted positively against yeast cells of Paracoccidioides sp., yeast cells of P. brasiliensis, and fungal cells of C. posadasii, while those derived from other strains were negative. The present study recorded for the first time the cross-reaction between the yeast cells of H. capsulatum and antibodies against Paracoccidioides spp., the yeast cells of Paracoccidioides sp. and antibodies against H. capsulatum, the yeast cells of Paracoccidioides sp. and antibodies against Coccidioides sp., and fungal cells of C. posadasii and antibodies against Paracoccidioides spp.

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http://dx.doi.org/10.1007/s11046-018-0295-0DOI Listing

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October 2018
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