Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Authors:
Tatiane Fernanda Sylvestre
Tatiane Fernanda Sylvestre
Tropical Diseases Department - Faculdade de Medicina de Botucatu - Universidade Estadual Paulista (UNESP)
Simone Schneider Weber
Simone Schneider Weber
Laboratório de Biologia Molecular
Bianca Alves Pauletti
Bianca Alves Pauletti
Centro Nacional de Pesquisa em Energia e Materiais
Brazil

PLoS One 2018 29;13(8):e0202804. Epub 2018 Aug 29.

Universidade Estadual Paulista (UNESP), Faculdade de Medicina de Botucatu, Botucatu, São Paulo, Brazil.

The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.

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Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202804PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114792PMC
February 2019
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References

(Supplied by CrossRef)
Aspectos epidemiológicos da paracoccidioidomicose na área endêmica de Botucatu (São Paulo-Brasil)
SA Marques et al.
Rev Inst Med Trop São Paulo 1983

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