Effects of Micronized Cartilage Matrix on Cartilage Repair in Osteochondral Lesions of the Talus.

Authors:
Alvin K Shieh
Alvin K Shieh
University of California
Tempe | United States
Sohni G Singh
Sohni G Singh
University of California Davis Medical Center
Connor Nathe
Connor Nathe
2 University of California Irvine School of Medicine
Evan Lian
Evan Lian
University of California
Prof. Dominik R Haudenschild, Ph.D.
Prof. Dominik R Haudenschild, Ph.D.
University of California Davis Medical Center
Professor
Arthritis Research
Sacramento, CA | United States
Jan A Nolta
Jan A Nolta
University of California
United States
Cassandra A Lee
Cassandra A Lee
Wake Forest University Baptist Medical Center
Eric Giza
Eric Giza
University of California Davis
United States

Cartilage 2018 Aug 29:1947603518796125. Epub 2018 Aug 29.

1 Department of Orthopaedic Surgery, University of California Davis Medical Center, Sacramento, CA, USA.

Background The repair of osteochondral lesions remains a challenge due to its poor vascularity and limited healing potential. Micronized cartilage matrix (MCM) is dehydrated, decellularized, micronized allogeneic cartilage matrix that contains the components of native articular tissue and is hypothesized to serve as a scaffold for the formation of hyaline-like tissue. Our objective was to demonstrate in vitro that the use of MCM combined with mesenchymal stem cells (MSCs) can lead to the formation of hyaline-like cartilage tissue in a single-stage treatment model. Design In group 1 (no wash), 250 µL MCM was reconstituted in 150 µL Dulbecco's phosphate-buffered saline (DPBS) for 5 minutes. Group 2 (saline wash) included 250 µL MCM washed in 20 mL DPBS for 30 minutes, then aspirated to remove all DPBS and reconstituted in 150 µL DPBS. Group 3 (serum wash): 250µL MCM washed in 20 mL DPBS for 30 minutes, then aspirated and reconstituted in 150 µL fetal bovine serum. Each group was then added to 50 µL solution of MSC suspended in DPBS at a concentration of 1.2 × 10 cells/350 µL. After 3 weeks, the defects were extracted and sectioned to perform viability and histologic analyses. Results Stem cells without rehydration of the MCM showed almost no viability whereas near complete cell viability was seen after rehydration with serum or saline solution, ultimately leading to chondrogenic differentiation and adhesion to the MCM particles. Conclusion We have shown in this proof-of-concept in vitro study that MCM can serve as a scaffold for the growth of cartilage tissue for the treatment of osteochondral lesions.

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August 2018
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References

(Supplied by CrossRef)
Article in J Bone Joint Surg Am
Knutsen G et al.
J Bone Joint Surg Am 2004

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