Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer.

Cell Physiol Biochem 2018 23;49(1):271-281. Epub 2018 Aug 23.

Department of Radiology, Ningbo No. 2 Hospital, Ningbo, China.

Background/aims: Smart molecular probes are required in the application of Magnetic resonance imaging (MRI) for biochemical and clinical research. This study aims to investigate the diagnostic values of estrogen receptor (ER), progesterone receptor (PR), folate receptor (FR) and human epidermal growth factor receptor 2 (HER-2)-targeted molecular probes in the MRI diagnosis of breast cancer.

Methods: Initially, a total of 508 female breast cancer patients were selected for breast cancer subtype classification by immunohistochemistry. Subsequently, the tumor size, lymph node metastasis, and histological grade of different breast cancer subtypes were compared. Molecular probes of Ab-ER-USPIO, Ab-PR-USPIO, Ab-FR-USPIO and Ab-HER-2-USPIO were constructed and screened. The specific binding of molecular probes to breast cancer cells was detected both in vitro and in vivo by Prussian blue staining and MRI using T1 and T2 weighted images. Finally, in vivo toxicity of Ab-HER-2-USPIO was analyzed using hematoxylin and eosin staining.

Results: We identified the following subtypes of breast cancer: Luminal A (ER-positive, FR-positive, HER-2-negative), Luminal B (ER-positive, FR-positive, HER-2-positive), HER-2 overexpression (ER-negative, FR-negative, HER-2-positive), and triple-negative breast cancer (ER-negative, FR-negative, HER-2-negative). Featuring favorable in vitro biocompatibility and low in vivo toxicity, Ab-HER-2-USPIO can specifically bind to breast cancer cells BT47 and SKBR3, thus enhancing the quality of T1 weighted MRI images.

Conclusion: The results indicate that HER-2-targeted MRI molecular probes may be used in the clinical diagnosis of breast cancer and facilitate the development of promising strategies for breast cancer treatments.

Download full-text PDF

Source
http://dx.doi.org/10.1159/000492877DOI Listing
September 2018
17 Reads

Publication Analysis

Top Keywords

breast cancer
40
molecular probes
24
breast
11
cancer
10
diagnosis breast
8
cancer cells
8
er-negative fr-negative
8
toxicity ab-her-2-uspio
8
vivo toxicity
8
resonance imaging
8
magnetic resonance
8
her-2-targeted molecular
8
er-positive fr-positive
8
luminal er-positive
8
molecular
6
probes
6
mri
5
mri weighted
4
staining mri
4
vivo prussian
4

Similar Publications