MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway.

J Exp Clin Cancer Res 2018 Aug 13;37(1):189. Epub 2018 Aug 13.

Department of Pathology, First Affiliated Hospital College and of Basic Medical Sciences China Medical University, No. 155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China.

Background: Makorin RING zinc finger-2 (MKRN2) belongs to the makorin RING zinc finger family and is a novel ubiquitin E3 ligase targeting the p65 subunit of NF-κB to negatively regulate inflammatory responses; however, the relationship between MKRN2 and tumorigenesis remains unclear. In this study, we clarified the role of MKRN2 in non-small cell lung cancer (NSCLC).

Methods: Tumor specimens collected from 261 NSCLC patients from 2013 to 2017 were retrieved from the Pathology Archive of the First Affiliated Hospital of China Medical University, and we performed assays to evaluate MKRN2 expression and to determine the impact of MKRN2 silencing and overexpression on NSCLC-cell migration and invasion.

Results: We demonstrated that MKRN2 expression was associated with lymph node metastasis, p-TNM stage, cancer-cell differentiation, and poor prognosis. By altering the expression of MKRN2 in selected cell lines, we found that MKRN2 inhibited cell migration and invasion through downregulation of the PI3K/Akt pathway.

Conclusions: These results suggested that MKRN2 inhibited NSCLC progression by reducing the metastatic potential of cancer cells. Our findings provide critical insight into the association of MKRN2 expression with favorable clinicopathological characteristics in NSCLC patients and suggested that MKRN2 plays a role in inhibiting NSCLC development.

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http://dx.doi.org/10.1186/s13046-018-0855-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090690PMC
August 2018
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References

(Supplied by CrossRef)

A Jemal et al.
CA Cancer J Clin 2010

RL Siegel et al.
CA Cancer J Clin 2016

LA Torre et al.
CA Cancer J Clin 2015

WD Travis et al.
Clin Chest Med 2002

A Jemal et al.
CA Cancer J Clin 2009

JD Minna et al.
Cancer Cell 2002

DA Fruman et al.
Nat Rev Drug Discov 2014

M Saji et al.
Biochem Biophys Res Commun 2005

BD Manning et al.
Cell 2007

I Hers et al.
Cell Signal 2011

AM Martelli et al.
Biochim Biophys Acta 1823

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