Development of customizable biomolecule functionalized 3D polycaprolactone (PCL) scaffolds reinforced with carbon nanofibers (CNF) for human meniscal tissue engineering

Janarthanan Gopinathan, Mamatha Muraleedharan Pillai, Sivanandam Shanthakumari, Singaram Gnanapoongothai, Beliyur Krishna Dinakar Rai, Kulasekaran Santosh Sahanand, Rajendran Selvakumar, Amitava Bhattacharyya

Overview

We developed customizable biomolecule functionalized 3D poly caprolactone (PCL) scaffolds reinforced with carbon nanofibers (CNF) for human meniscal tissue engineering. Highly porous, electrically conductive 3D nanocomposite scaffolds exhibited porous nanoarchitectures which facilitated enhanced mechanical integrity and meniscus cell augmentation in vitro. In vivo biocompatibility studies on rabbits show admirable results

Summary

we envisioned to investigate and explore the possibility of using CNF with multiple properties in developing porous 3D polymeric scaffolds with high mechanical integrity intended for meniscal tissue engineering.

Author Comments

Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India
Writing this article was a great pleasure as it has co-authors with whom I have had long standing collaborations. Dr. Mamatha M Pillai, PhD

Resources

Carbon nanofiber amalgamated 3D poly-ε-caprolactone scaffold functionalized porous-nanoarchitectures for human meniscal tissue engineering: In vitro and in vivo biocompatibility studies.

Authors:
Dr Janarthanan Gopinathan, PhD
Dr Janarthanan Gopinathan, PhD
Seoul National University of Science and Technology
Post Doctoral Research Scientist
Nowon-gu, Seoul | Korea, Republic of
Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India

Nanomedicine 2018 10 4;14(7):2247-2258. Epub 2018 Aug 4.

Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Peelamedu, Coimbatore, India; Department of Electronics and Communication Engineering, PSG College of Technology, Coimbatore, India. Electronic address:

We developed customizable biomolecule functionalized 3D poly-ε-caprolactone (PCL) scaffolds reinforced with carbon nanofibers (CNF) for human meniscal tissue engineering. 3D nanocomposite scaffolds exhibited commendable mechanical integrity and electrical properties with augmented cytocompatibility. Especially, the functionalized 3D (10wt% CNF) scaffolds showed ~363% increase in compressive moduli compared to the pristine PCL. In dynamic mechanical analysis, these scaffolds achieved highest value (~42 MPa at 10 Hz) among all tested scaffolds including pristine PCL and human menisci (33, 41, 56 years). In vitro results were well supported by the outcomes of cell proliferation analysis, microscopic images, Hoechst staining and extracellular-matrix estimation. Further, in vivo rabbit bio toxicity studies revealed scaffold's non-toxicity and its future potential as a meniscus scaffold. This study also indicates that the incorporation of CNF in polymer matrix may be optimized based on mechanical properties of patient meniscus and it may help in developing the customized patient specific 3D constructs with improved multifunctional properties.

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http://dx.doi.org/10.1016/j.nano.2018.07.012DOI Listing
October 2018
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