Biotechnol Lett 2018 Oct 30;40(9-10):1395-1406. Epub 2018 Jul 30.
Laboratório de Enzimologia, Departamento de Biologia Celular, Instituto Central de Ciências Sul, Universidade de Brasília - UnB, Brasília, DF, 700910-900, Brazil.
Objective: To isolate putative lipase enzymes by screening a Cerrado soil metagenomic library with novel features.
Results: Of 6720 clones evaluated, Clone W (10,000 bp) presented lipolytic activity and four predicted coding sequences, one of them LipW. Characterization of a predicted esterase/lipase, LipW, showed 28% sequence identity with an arylesterase from Pseudomonas fluorescens (pdb|3HEA) from protein database (PDB). Phylogenetic analysis showed LipW clustered with family V lipases; however, LipW was clustered in different subclade belonged to family V, suggesting a different subgroup of family V. In addition, LipW presented a difference in family V GH motif, a glycine replaced by a serine in GH motif. Estimated molecular weight and stokes radius values of LipW were 29,338.67-29,411.98 Da and 2.58-2.83 nm, respectively. Optimal enzyme activity was observed at pH 9.0-9.5 and at 40 °C. Circular dichroism analysis estimated secondary structures percentages as approximately 45% α-helix and 15% β-sheet, consistent with the 3D structure predicted by homology.
Conclusion: Our results demonstrate the isolation of novel family V lipolytic enzyme with biotechnological applications from a metagenomic library.