Cytoskeletal Mechanisms of Axonal Contractility.

Authors:
Sampada P Mutalik
Sampada P Mutalik
Indian Institute of Science Education and Research Pune
Pramod A Pullarkat
Pramod A Pullarkat
Raman Research Institute
Bengaluru | India
Aurnab Ghose
Aurnab Ghose
Harvard Medical School
United States

Biophys J 2018 Aug 12;115(4):713-724. Epub 2018 Jul 12.

Indian Institute of Science Education and Research Pune, Pune, Maharashtra, India. Electronic address:

Mechanotransduction is likely to be an important mechanism of signaling in thin, elongated cells such as neurons. Maintenance of prestress or rest tension may facilitate mechanotransduction in these cells. In recent years, functional roles for mechanical tension in neuronal development and physiology are beginning to emerge, but the cellular mechanisms regulating neurite tension remain poorly understood. Active contraction of neurites is a potential mechanism of tension regulation. In this study, we have explored cytoskeletal mechanisms mediating active contractility of neuronal axons. We have developed a simple assay in which we evaluate contraction of curved axons upon trypsin-mediated detachment. We show that curved axons undergo contraction and straighten upon deadhesion. Axonal straightening was found to be actively driven by actomyosin contractility, whereas microtubules may subserve a secondary role. We find that although axons show a monotonous decrease in length upon contraction, subcellularly, the cytoskeleton shows a heterogeneous contractile response. Further, using an assay for spontaneous development of tension without trypsin-induced deadhesion, we show that axons are intrinsically contractile. These experiments, using novel experimental approaches, implicate the axonal cytoskeleton in tension homeostasis. Our data suggest that although globally, the axon behaves as a mechanical continuum, locally, the cytoskeleton is remodeled heterogeneously.

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Cytoskeletal Mechanisms of Axonal Contractility


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http://dx.doi.org/10.1016/j.bpj.2018.07.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104292PMC

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August 2018
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