Identification of differentially expressed proteins in the gastric mucosal atypical hyperplasia tissue microenvironment.

Oncol Lett 2018 Aug 11;16(2):2355-2365. Epub 2018 Jun 11.

Key Laboratory of Cancer Cellular and Molecular Pathology, Cancer Research Institute of Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.

In the present study, the interaction of proteins in the microenvironment of gastric mucosal atypical hyperplasia was analyzed. The stromata of normal gastric mucosa (NGM) and gastric mucosal atypical hyperplasia (GMAH) tissues were purified with laser capture microdissection (LCM). The differentially expressed GMAH proteins of the NGM and GMAH tissues were identified by quantitative proteomic techniques with isotope labeling. The cross-talk between differentially expressed proteins in NGM and GMAH tissues was then analyzed by bioinformatics. There were 165 differentially expressed proteins identified from the stromata of NGM and GMAH tissues. Among them, 99 proteins were upregulated and 66 were downregulated in GMAH tissue. The present study demonstrated that these proteins in gastric mucosal atypical hyperplasia were involved in cancer-associated signaling pathways, including the p53, mitogen-activated protein kinase (MAPK), cell cycle and apoptosis signaling pathways, and were involved in cellular growth, cellular proliferation, apoptosis and the humoral immune response. The results of the present study suggest that the 165 differentially expressed proteins, including S100 calcium-binding protein A6 (S100A6) and superoxide dismutase 3 (SOD3) in the microenvironment of gastric mucosal atypical hyperplasia, are involved in the p53, MAPK, cell cycle and apoptosis signaling pathways, and serve a function in the pathogenesis of gastric cancer.

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2018.8941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036401PMC
August 2018
7 Reads

Publication Analysis

Top Keywords

gastric mucosal
20
differentially expressed
20
mucosal atypical
20
atypical hyperplasia
20
expressed proteins
16
gmah tissues
16
signaling pathways
12
ngm gmah
12
mapk cell
8
hyperplasia involved
8
proteins ngm
8
cell cycle
8
microenvironment gastric
8
cycle apoptosis
8
165 differentially
8
proteins
8
proteins gastric
8
apoptosis signaling
8
gastric
7
gmah
6

Similar Publications