Toxoplasma Parasite Twisting Motion Mechanically Induces Host Cell Membrane Fission to Complete Invasion within a Protective Vacuole.

Authors:
Georgios Pavlou
Georgios Pavlou
Institute for Advanced Biosciences (IAB)
Mateusz Biesaga
Mateusz Biesaga
Institute for Advanced Biosciences (IAB)
Bastien Touquet
Bastien Touquet
France; Université Joseph Fourier
Vanessa Lagal
Vanessa Lagal
Albert Einstein College of Medicine
United States
Martial Balland
Martial Balland
Univ. Grenoble 1/CNRS
France
Alexandre Dufour
Alexandre Dufour
Institut Pasteur
Mohamed-Ali Hakimi
Mohamed-Ali Hakimi
The Wistar Institute
United States
Isabelle Tardieux
Isabelle Tardieux
Université Paris Descartes
France

Cell Host Microbe 2018 07 28;24(1):81-96.e5. Epub 2018 Jun 28.

Institute for Advanced Biosciences (IAB), Team Membrane Dynamics of Parasite-Host Cell Interactions, CNRS UMR5309, INSERM U1209, Université Grenoble Alpes, Grenoble 38700, France. Electronic address:

To invade cells, the parasite Toxoplasma gondii injects a multi-unit nanodevice into the target cell plasma membrane (PM). The core nanodevice, which is composed of the RhOptry Neck (RON) protein complex, connects Toxoplasma and host cell through a circular tight junction (TJ). We now report that this RON nanodevice mechanically promotes membrane scission at the TJ-PM interface, directing a physical rotation driven by the parasite twisting motion that enables the budding parasitophorous vacuole (PV) to seal and separate from the host cell PM as a bona fide subcellular Toxoplasma-loaded PV. Mechanically impairing the process induces swelling of the budding PV and death of the parasite but not host cell. Moreover, this study reveals that the parasite nanodevice functions as a molecular trigger to promote PV membrane remodeling and rapid onset of T. gondii to intracellular lifestyle.

Abstract Video

T. gondii Requires the Twisting Motion Driven by the RON Protein Complex


Source: Cell Press

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http://dx.doi.org/10.1016/j.chom.2018.06.003DOI Listing

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July 2018
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