A Multiagent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus-Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges.

Authors:
Lesley C Dupuy
Lesley C Dupuy
United States Army Medical Research Institute of Infectious Diseases
United States
Brian D Livingston
Brian D Livingston
Ichor Medical Systems
San Diego | United States
Drew Hannaman
Drew Hannaman
Ichor Medical Systems
San Diego | United States
Connie S Schmaljohn
Connie S Schmaljohn
United States Army Medical Research Institute of Infectious Diseases
United States

J Immunol Res 2018 3;2018:8521060. Epub 2018 Jun 3.

United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.

There remains a need for vaccines that can safely and effectively protect against the biological threat agents Venezuelan (VEEV), western (WEEV), and eastern (EEEV) equine encephalitis virus. Previously, we demonstrated that a VEEV DNA vaccine that was optimized for increased antigen expression and delivered by intramuscular (IM) electroporation (EP) elicited robust and durable virus-specific antibody responses in multiple animal species and provided complete protection against VEEV aerosol challenge in mice and nonhuman primates. Here, we performed a comparative evaluation of the immunogenicity and protective efficacy of individual optimized VEEV, WEEV, and EEEV DNA vaccines with that of a 1 : 1 : 1 mixture of these vaccines, which we have termed the 3-EEV DNA vaccine, when delivered by IM EP. The individual DNA vaccines and the 3-EEV DNA vaccine elicited robust and durable virus-specific antibody responses in mice and rabbits and completely protected mice from homologous VEEV, WEEV, and EEEV aerosol challenges. Taken together, the results from these studies demonstrate that the individual VEEV, WEEV, and EEEV DNA vaccines and the 3-EEV DNA vaccine delivered by IM EP provide an effective means of eliciting protection against lethal encephalitic alphavirus infections in a murine model and represent viable next-generation vaccine candidates that warrant further development.

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Source
http://dx.doi.org/10.1155/2018/8521060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008678PMC
October 2018
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References

(Supplied by CrossRef)

2001
Article in Infectious Disease Clinics of North America
Infectious Disease Clinics of North America 1991

2007
Article in Canadian Medical Association Journal
Canadian Medical Association Journal 1968
Article in Clinics in Laboratory Medicine
Clinics in Laboratory Medicine 2001
Article in Journal of Environmental Health
Journal of Environmental Health 2001

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