Extractive purification of recombinant thermostable lipase from fermentation broth of using an aqueous polyethylene glycol impregnated resin system.

3 Biotech 2018 Jun 9;8(6):288. Epub 2018 Jun 9.

6School of Engineering, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Selangor Malaysia.

This study aimed at recovery of thermostable lipase from BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-018-1295-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994206PMC
June 2018
10 Reads

Publication Analysis

Top Keywords

polyethylene glycol
8
porous glass
8
purification factor
8
thermostable lipase
8
glass beads
8
lipase purified
4
purified airs
4
influencing parameters
4
parameters concentration
4
extraction solution
4
adsorbent recombinant
4
concentration extraction
4
recombinant lipase
4
airs purification
4
yield 784%
4
aqueous impregnated
4
peg aqueous
4
impregnated resins
4
resins system
4
solution concentration
4

Similar Publications