Pubfacts - Scientific Publication Data
  • Categories
  • Journals
  • ->
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Outcomes of Measurable Residual Disease in Pediatric Acute Myeloid Leukemia before and after Hematopoietic Stem Cell Transplant: Validation of Difference from Normal Flow Cytometry with Chimerism Studies and Wilms Tumor 1 Gene Expression.

Authors:
David A Jacobsohn Michael R Loken Mingwei Fei Alexia Adams Lisa Eidenschink Brodersen Brent R Logan Kwang Woo Ahn Bronwen E Shaw Morris Kletzel Marie Olszewski Sana Khan Soheil Meshinchi Amy Keating Andrew Harris Pierre Teira Reggie E Duerst Steven P Margossian Paul L Martin Aleksandra Petrovic Christopher C Dvorak Eneida R Nemecek Michael W Boyer Allen R Chen Jeffrey H Davis Shalini Shenoy Sureyya Savasan Michelle P Hudspeth Roberta H Adams Victor A Lewis Albert Kheradpour Kimberly A Kasow Alfred P Gillio Ann E Haight Monica Bhatia Barbara J Bambach Hilary L Haines Troy C Quigg Robert J Greiner Julie-An M Talano David C Delgado Alexandra Cheerva Madhu Gowda Sanjay Ahuja Mehmet Ozkaynak David Mitchell Kirk R Schultz Terry J Fry David M Loeb Michael A Pulsipher

Biol Blood Marrow Transplant 2018 10 19;24(10):2040-2046. Epub 2018 Jun 19.

Division of Hematology, Oncology, and Blood and Marrow Transplantation, Children's Hospital Los Angeles, USC Keck School of Medicine, Los Angeles, CA, USA.

We enrolled 150 patients in a prospective multicenter study of children with acute myeloid leukemia undergoing hematopoietic stem cell transplantation (HSCT) to compare the detection of measurable residual disease (MRD) by a "difference from normal" flow cytometry (ΔN) approach with assessment of Wilms tumor 1 (WT1) gene expression without access to the diagnostic specimen. Prospective analysis of the specimens using this approach showed that 23% of patients screened for HSCT had detectable residual disease by ΔN (.04% to 53%). Of those patients who proceeded to transplant as being in morphologic remission, 10 had detectable disease (.04% to 14%) by ΔN. The disease-free survival of this group was 10% (0 to 35%) compared with 55% (46% to 64%, P < .001) for those without disease. The ΔN assay was validated using the post-HSCT specimen by sorting abnormal or suspicious cells to confirm recipient or donor origin by chimerism studies. All 15 patients who had confirmation of tumor detection relapsed, whereas the 2 patients with suspicious phenotype cells lacking this confirmation did not. The phenotype of the relapse specimen was then used retrospectively to assess the pre-HSCT specimen, allowing identification of additional samples with low levels of MRD involvement that were previously undetected. Quantitative assessment of WT1 gene expression was not predictive of relapse or other outcomes in either pre- or post-transplant specimens. MRD detected by ΔN was highly specific, but did not identify most relapsing patients. The application of the assay was limited by poor quality among one-third of the specimens and lack of a diagnostic phenotype for comparison.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbmt.2018.06.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6239928PMC
October 2018

Publication Analysis

Top Keywords

residual disease
12
gene expression
12
wilms tumor
8
stem cell
8
myeloid leukemia
8
hematopoietic stem
8
wt1 gene
8
acute myeloid
8
flow cytometry
8
measurable residual
8
disease Δn
8
chimerism studies
8
patients
6
disease
5
Δn
5
abnormal suspicious
4
post-hsct specimen
4
assay validated
4
validated post-hsct
4
suspicious cells
4

Similar Publications

Changes in SARS CoV-2 Seroprevalence Over Time in Ten Sites in the United States, March - August, 2020.

Authors:
Travis Lim Mark Delorey Nicolette Bestul Michael Johannsen Carrie Reed Aron J Hall Alicia M Fry Chris Edens Vera Semenova Han Li Peter Browning Rita Desai Monica Epperson Tao Jia Natalie J Thornburg Jarad Schiffer Fiona P Havers

Clin Infect Dis 2021 Feb 26. Epub 2021 Feb 26.

U.S. Centers for Disease Control and Prevention COVID-19 Response Team, Atlanta, Georgia, United States.

Background: Monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody prevalence can complement case reporting to inform more accurate estimates of SARS-CoV-2 infection burden, but few studies have undertaken repeated sampling over time on a broad geographic scale.

Methods: We performed serologic testing on a convenience sample of residual sera obtained from persons of all ages, at ten sites in the United States from March 23 through August 14, 2020, from routine clinical testing at commercial laboratories. We age-sex-standardized our seroprevalence rates using census population projections and adjusted for laboratory assay performance. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Outcomes for Australian children with relapsed/refractory acute lymphoblastic leukaemia treated with blinatumomab.

Authors:
Rosemary Sutton Luciano Dalla Pozza Seong Lin Khaw Chris Fraser Tom Revesz Janis Chamberlain Richard Mitchell Toby N Trahair Caroline M Bateman Nicola C Venn Tamara Law Erika Ong Susan L Heatley Barbara J McClure Claus Meyer Rolf Marschalek Michelle J Henderson Siobhan Cross Deborah L White Rishi S Kotecha

Pediatr Blood Cancer 2021 Feb 26:e28922. Epub 2021 Feb 26.

Department of Clinical Haematology, Oncology and Bone Marrow Transplantation, Perth Children's Hospital, Perth, Western Australia, Australia.

We report on the Australian experience of blinatumomab for treatment of 24 children with relapsed/refractory precursor B-cell acute lymphoblastic leukaemia (B-ALL) and high-risk genetics, resulting in a minimal residual disease (MRD) response rate of 58%, 2-year progression-free survival (PFS) of 39% and 2-year overall survival of 63%. In total, 83% (n = 20/24) proceeded to haematopoietic stem cell transplant, directly after blinatumomab (n = 12) or following additional salvage therapy (n = 8). Four patients successfully received CD19-directed chimeric antigen receptor T-cell therapy despite prior blinatumomab exposure. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Clinical impact of PCSK9 inhibitor on stabilization and regression of lipid-rich coronary plaques: a near-infrared spectroscopy study.

Authors:
Hideaki Ota Hiroyuki Omori Masanori Kawasaki Akihiro Hirakawa Hitoshi Matsuo

Eur Heart J Cardiovasc Imaging 2021 Feb 26. Epub 2021 Feb 26.

Department of Cardiology, Gifu Heart Center, 4-14-4 Yabuta-minami, Gifu 500-8384, Japan.

Aims: This study aimed to determine the effects of a proprotein convertase subtilisin-kexin type 9 inhibitor (PCSK9i) on coronary plaque volume and lipid components in patients with a history of coronary artery disease (CAD).

Methods And Results: This prospective, open-label, single-centre study analysed non-culprit coronary segments using near-infrared spectroscopy-intravascular ultrasound (NIRS-IVUS) at baseline and follow-up angiography. Following changes in the lipid-lowering treatment based on the most recent guideline, the enrolled subjects were divided into two groups: treatment with PCSK9i and statins (PCSK9i: 21 patients and 40 segments) and statins only (control: 32 patients and 50 segments). Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Genetic spectrum and clinical early natural history of glucose-6-phosphate dehydrogenase deficiency in Mexican children detected through newborn screening.

Authors:
Marcela Vela-Amieva Miguel Angel Alcántara-Ortigoza Ariadna González-Del Angel Leticia Belmont-Martínez Carlos López-Candiani Isabel Ibarra-González

Orphanet J Rare Dis 2021 Feb 26;16(1):103. Epub 2021 Feb 26.

UGN, Instituto de Investigaciones Biomédicas, UNAM-LEIMyT, Instituto Nacional de Pediatría SS, CDMX, Mexico.

Background: Glucose-6-phosphate dehydrogenase deficiency (G6PDd) newborn screening is still a matter of debate due to its highly heterogeneous birth prevalence and clinical expression, as well as, the lack of enough knowledge on its natural history. Herein, we describe the early natural clinical course and the underlying GDPD genotypes in infants with G6PDd detected by newborn screening and later studied in a single follow-up center. G6PDd newborns were categorized into three groups: group 1: hospitalized with or without neonatal jaundice (NNJ); group 2: non-hospitalized with NNJ; and group 3: asymptomatic. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Margin Assessment and Re-excision Rates for Patients Who Have Neoadjuvant Chemotherapy and Breast-Conserving Surgery.

Authors:
Cindy Cen Jennifer Chun Elianna Kaplowitz Deborah Axelrod Richard Shapiro Amber Guth Freya Schnabel

Ann Surg Oncol 2021 Feb 26. Epub 2021 Feb 26.

Department of Surgery, New York University Langone Health, New York, NY, USA.

Background: Neoadjuvant chemotherapy (NAC) has enabled more patients to be eligible for breast-conservation surgery (BCS). Achieving negative lumpectomy margins, however, is challenging due to changes in tissue composition and potentially scattered residual carcinoma in the tumor bed. Data regarding BCS after NAC have shown variable re-excision rates. Read More

View Article and Full-Text PDF
February 2021
Similar Publications
Get 20% Off Journals at LWW.com
© 2021 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap