Direct recovery of mangostins from Garcinia mangostana pericarps using cellulase-assisted aqueous micellar biphasic system with recyclable surfactant.

J Biosci Bioeng 2018 Oct 12;126(4):507-513. Epub 2018 Jun 12.

Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, 135 Yuan Tung Road, Chungli, Taoyuan 320, Taiwan. Electronic address:

The α- and γ-mangostins from Garcinia mangostana pericarps (GMP) exhibit antioxidant, anti-bacterial, anti-inflammatory and anti-tumor properties. The extraction yields α- and γ-mangostins are often limited by the presence of the GMP cell walls. Therefore, the extraction and recovery of mangostins from GMP with an Aspergillus niger cellulase-assisted aqueous micellar biphasic system (CA-AMBS) was developed for enhanced yield of mangostins. Effects of the concentration of cellulase, the incubation time and the temperature of the system on the recovery of mangostins were investigated. The optimum condition for the recovery of α- and γ-mangostins was obtained with the addition of 0.5% (w/w) cellulase incubated at 40°C for 2 h. High log partition coefficients of α-mangostins (log K 4.79 ± 0.02) and γ-mangostins (log K 4.02 ± 0.02) were achieved. High yields of α-mangostins (73.4%) and γ-mangostins (14.0%) were obtained from the micelle-rich bottom phase with final concentrations of 3.67 mg/mL and 0.70 mg/mL, respectively. The back-extraction of mangostins was performed with the addition of 30% (w/w) of isopropanol and 0.05 M of KCl at pH 9 to the bottom phase of the CA-AMBS. The yields of the α- and γ-mangostins from GMP were considerably enhanced with the CA-AMBS and the direct recovery of mangostins was demonstrated without additional downstream processing steps.

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S13891723183015
Publisher Site
http://dx.doi.org/10.1016/j.jbiosc.2018.04.008DOI Listing
October 2018
51 Reads

Publication Analysis

Top Keywords

α- γ-mangostins
16
recovery mangostins
16
bottom phase
8
biphasic system
8
aqueous micellar
8
mangostana pericarps
8
micellar biphasic
8
cellulase-assisted aqueous
8
yields α-
8
garcinia mangostana
8
direct recovery
8
γ-mangostins
6
mangostins
6
log partition
4
partition coefficients
4
high log
4
2 h high
4
log 402 ± 002
4
40°c 2 h
4
coefficients α-mangostins
4

Similar Publications