Non-tuberculous Mycobacterium species causing mycobacteriosis in farmed aquatic animals of South Africa.

BMC Microbiol 2018 04 13;18(1):32. Epub 2018 Apr 13.

Tuberculosis Laboratory, Agricultural Research Council - Onderstepoort Veterinary Research, Onderstepoort, South Africa.

Background: Mycobacteriosis caused by non-tuberculous mycobacteria (NTM), is among the most chronic diseases of aquatic animals. In addition, fish mycobacteriosis has substantial economic consequences especially in the aquaculture and fisheries industry as infections may significantly decrease production and trade. Some fish NTM pathogens are highly virulent and zoonotic; as such, infection of aquaria with these pathogens is a public health concern. In this study, we report isolation of nine different NTM species from sixteen aquatic animals including different fish species, frogs and a crocodile. Given the clinical significance of Mycobacterium marinum and its close relation to Mycobacterium tuberculosis, as well as the significance of ESAT 6 and CFP-10 secretion in mycobacterial virulence, we analysed the esxA and esxB nucleotide sequences of M. marinum isolates identified in this study as well as other mycobacteria in the public databases.

Results: Mycobacterium shimoidei, Mycobacterium marinum, Mycobacterium chelonae, Mycobacterium septicum /M. peregrinum and Mycobacterium porcinum were isolated from gold fish, Guppy, exotic fish species in South Africa, koi and undefined fish, Knysna seahorse, as well Natal ghost frogs respectively, presenting tuberculosis like granuloma. Other NTM species were isolated from the studied aquatic animals without any visible lesions, and these include Mycobacterium sp. N845 T, Mycobacterium fortuitum, a member of the Mycobacterium avium complex, and Mycobacterium szulgai. Phylogenetic analysis of mycobacteria, based on esxA and esxB genes, separated slow growing from rapidly growing mycobacteria as well as pathogenic from non-pathogenic mycobacteria in some cases.

Conclusions: Isolation of the different NTM species from samples presenting granuloma suggests the significance of these NTM species in causing mycobacteriosis in these aquatic animals. The study also revealed the potential of esxA and esxB sequences as markers for phylogenetic classification of mycobacteria. Observations regarding use of esxA and esxB sequences for prediction of potential pathogenicity of mycobacteria warrants further investigation of these two genes in a study employing NTM species with well-defined pathogenicity.

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https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s128
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http://dx.doi.org/10.1186/s12866-018-1177-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899368PMC

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April 2018
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References

(Supplied by CrossRef)
Article in J Fish Dis
L Novotny et al.
J Fish Dis 2010
Article in Vet Med
M Slany et al.
Vet Med 2010
Article in Infect Ecol Epidemiol
SJ Willson et al.
Infect Ecol Epidemiol 2013
Article in J Zoo Wildl Med
SJ Woodhouse et al.
J Zoo Wildl Med 2014
Article in J Comp Pathol
M Barrows et al.
J Comp Pathol 2017
Article in Vet Microbiol
A Decostere et al.
Vet Microbiol 2004
Article in Vet J
TD Gauthier et al.
Vet J 2009
Article in J Fish Dis
CB Stine et al.
J Fish Dis 2010
Article in J Vet Med Sci
K Nakanaga et al.
J Vet Med Sci 2012
Article in Lancet Infect Dis
V Sizaire et al.
Lancet Infect Dis 2006
Article in J Clin Microbiol
V Broutin et al.
J Clin Microbiol 2012

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