Use of TaqMan Array Cards to Screen Outbreak Specimens for Causes of Febrile Illness in Tanzania.

Authors:
Dr Ahmed Abade Mohamed, PhD
Dr Ahmed Abade Mohamed, PhD
Tanzania Field Epidemiology and Laboratory Training Program
Medical Epidemiologist
infectious disease
Dar es Salaam, East Africa | Tanzania, United Republic of

Am J Trop Med Hyg 2018 06 29;98(6):1640-1642. Epub 2018 Mar 29.

University of Virginia, Charlottesville, Virginia.

We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015-2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks.

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Source
http://dx.doi.org/10.4269/ajtmh.18-0071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086183PMC
June 2018
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