Differential expression pattern of co-inhibitory molecules on CD4 T cells in uncomplicated versus complicated malaria.

Authors:
Dr Otchere Addai-Mensah
Dr Otchere Addai-Mensah
Kwame Nkrumah University of Science and Technology
Senior Lecturer and Dean of Faculty
Immunology and Malariology
Kumasi, Ashanti | Ghana

Sci Rep 2018 03 19;8(1):4789. Epub 2018 Mar 19.

I. Medical Department, Division of Tropical Medicine and Infectious Diseases, University Medical Centre Hamburg Eppendorf, 20246, Hamburg, Germany.

The immune response of malaria patients is a main factor influencing the clinical severity of malaria. A tight regulation of the CD4 T cell response or the induction of tolerance have been proposed to contribute to protection from severe or clinical disease. We therefore compared the CD4 T cell phenotypes of Ghanaian children with complicated malaria, uncomplicated malaria, asymptomatic Plasmodium falciparum (Pf) infection or no infection. Using flow cytometric analysis and automated multivariate clustering, we characterized the expression of the co-inhibitory molecules CTLA-4, PD-1, Tim-3, and LAG-3 and other molecules implicated in regulatory function on CD4 T cells. Children with complicated malaria had higher frequencies of CTLA-4 or PD-1 CD4 T cells than children with uncomplicated malaria. Conversely, children with uncomplicated malaria showed a higher proportion of CD4 T cells expressing CD39 and Granzyme B, compared to children with complicated malaria. In contrast, asymptomatically infected children expressed only low levels of co-inhibitory molecules. Thus, different CD4 T cell phenotypes are associated with complicated versus uncomplicated malaria, suggesting a two-sided role of CD4 T cells in malaria pathogenesis and protection. Deciphering the signals that shape the CD4 T cell phenotype in malaria will be important for new treatment and immunization strategies.

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-22659-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859076PMC
March 2018
21 Reads
5.080 Impact Factor

Publication Analysis

Top Keywords

cd4 cells
20
complicated malaria
16
uncomplicated malaria
16
cd4 cell
16
malaria
12
co-inhibitory molecules
12
children complicated
12
cd4
9
children uncomplicated
8
malaria higher
8
ctla-4 pd-1
8
cells children
8
molecules cd4
8
cell phenotypes
8
children
6
complicated
5
cells
5
uncomplicated
5
suggesting two-sided
4
automated multivariate
4

References

(Supplied by CrossRef)

PD Crompton et al.
Annual review of immunology 2014

M Walther et al.
J Immunol 2006

EM Riley et al.
Immunology today 1999

K Artavanis-Tsakonas et al.
Clinical and experimental immunology 2003

DJ Pombo et al.
Lancet 2002

H Xu et al.
The Journal of experimental medicine 2002

AP Freitas do Rosario et al.
J Immunol 2012

IR Okonkwo et al.
Journal of the Pediatric Infectious Diseases Society 2016

J Langhorne et al.
Nature immunology 2008

S Portugal et al.
PLoS pathogens 2014

Similar Publications