A survey of the truffle Tuber melanosporum genome has shown the presence of 67 programmed cell death (PCD)- related genes. The 67 genes are all expressed during fruit body (FB) development of T. melanosporum development; their expression has been detected by DNA microarrays and qPCR. A set of 14 PCD-related genes have been chosen, those with the highest identities to the homologs of other species, for a deeper investigation. That PCD occurs during T. melanosporum development has been demonstrated by the TUNEL reaction and transmission electron microscopy. The findings of this work, in addition to the discovery of PCD-related genes in the T. melanosporum genome and their expression during the differentiation and development of the FB, would suggest that one of the PCD subroutines, maybe autophagy, is involved in the FB ripening, i.e., sporogenesis.
The 67 genes are all expressed during fruit body (FB) development of T. melanosporum development; their expression has been detected by DNA microarrays and qPCR.
The complete expression dataset is available as series (GPL8982) at the Gene Expression Omnibus at NCBI (http://www.ncbi.nlm.nih.gov/geo/).Dr. Osvaldo Zarivi
Cell Death Discov 2018 Dec 20;4:32. Epub 2018 Feb 20.
1Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, 67100 Italy.
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