The genomic of truffle, programmed cell death.

Osvaldo Zarivi, Patrizia Cesare, Anna Maria Poma, Sabrina Colafarina, Antonella Bonfigli, Annegret Kohler, Pierpaolo Aimola, Anna Maria Ragnelli, Giovanni Pacioni, Michele Miranda

Overview

A survey of the truffle Tuber melanosporum genome has shown the presence of 67 programmed cell death (PCD)- related genes. The 67 genes are all expressed during fruit body (FB) development of T. melanosporum development; their expression has been detected by DNA microarrays and qPCR. A set of 14 PCD-related genes have been chosen, those with the highest identities to the homologs of other species, for a deeper investigation. That PCD occurs during T. melanosporum development has been demonstrated by the TUNEL reaction and transmission electron microscopy. The findings of this work, in addition to the discovery of PCD-related genes in the T. melanosporum genome and their expression during the differentiation and development of the FB, would suggest that one of the PCD subroutines, maybe autophagy, is involved in the FB ripening, i.e., sporogenesis.

Summary

The 67 genes are all expressed during fruit body (FB) development of T. melanosporum development; their expression has been detected by DNA microarrays and qPCR.

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Author Comments

Dr. Osvaldo Zarivi
Dr. Osvaldo Zarivi
University of L'Aquila
Dip.MeSVA
L'Aquila | Italy
The complete expression dataset is available as series (GPL8982) at the Gene Expression Omnibus at NCBI (http://www.ncbi.nlm.nih.gov/geo/).Dr. Osvaldo Zarivi

Resources

https://www.nature.com/articles/s41420-017-0019-0.pdf?origin=ppub
https://www.nature.com/articles/s41420-017-0019-0.pdf?origin=ppub

The genomic tool-kit of the truffle programmed cell death.

Cell Death Discov 2018 Dec 20;4:32. Epub 2018 Feb 20.

1Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, 67100 Italy.

A survey of the truffle genome has shown the presence of 67 programmed cell death (PCD)-related genes. The 67 genes are all expressed during fruit body (FB) development of development; their expression has been detected by DNA microarrays and qPCR. A set of 14 PCD-related genes have been chosen, those with the highest identities to the homologs of other species, for a deeper investigation. That PCD occurs during development has been demonstrated by the TUNEL reaction and transmission electron microscopy. The findings of this work, in addition to the discovery of PCD-related genes in the genome and their expression during the differentiation and development of the FB, would suggest that one of the PCD subroutines, maybe autophagy, is involved in the FB ripening, i.e., sporogenesis.

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Source
http://dx.doi.org/10.1038/s41420-017-0019-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841409PMC
December 2018
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