The association of endothelial nitric oxide synthase gene single nucleotide polymorphisms with paediatric systemic lupus erythematosus.

Authors:
Jia Zhu
Jia Zhu
Tangdu Hospital
China
Dr. Zhen Wang, MD,PhD
Dr. Zhen Wang, MD,PhD
Shanghai Tenth People’s Hospital, Tongji University School of Medicine
Professor
Nephrology
Shanghai | China
Guowei Song
Guowei Song
Nanjing University of Technology
China
Ting Zhang
Ting Zhang
Capital Institute of Pediatrics
China
MRs Li Wang, RN
MRs Li Wang, RN
shepherd university
RN
RN
walnut , CA | United States

Clin Exp Rheumatol 2018 May-Jun;36(3):508-512. Epub 2018 Jan 31.

Division of Paediatric Rheumatology, The Affiliated Children's Hospital, Capital Institute of Paediatrics, Beijing, China.

Objectives: Endothelial nitric oxide synthase (eNOS) is a type of nitric oxide synthase that mainly exists in the endothelium. It produces nitric oxide (NO) to regulate the function of endothelial cells. Endothelial dysfunction and increased NO metabolites have been shown in animal models of lupus and in lupus patients, so eNOS gene polymorphisms may be important in the pathogenesis of SLE. This study aimed to investigate the association of the single nucleotide polymorphisms (SNPs) of eNOS and paediatric systemic lupus erythematosus (pSLE).

Methods: A total of 91 pSLE cases and 90 healthy controls were used in this study. We divided these patients into 4 subgroups according to kidney or central nervous system involvement. Four selected SNPs in the gene were analysed with MALDI-TOF mass spectrometry. Statistical methods were carried out to investigate the correlation between the SNPs and pSLE.

Results: SNP rs1808593 genotype GT in case group were significantly higher than those in the control group (p=0.047), and the genotype GT had positive correlation with pSLE (OR=1.93, 95% CI: 1.01-3.69). In subgroup C (the patients with central nervous system but no kidney damage), the genotype GT was significantly higher than those in the control group (p=0.028), and the genotype GT was related to pSLE with central nervous system damage (OR=6.24, 95% CI: 1.17-33.15). In male patients, we found SNP rs1808593 genotype GT in pSLE group was significantly higher than in the control group (p=0.0065), and the risk of pSLE increased in patients with genotype GT (OR=8.36, 95% CI: 2.02-34.6).

Conclusions: SNP rs1808593 GT genotype is significantly higher than that in the control group, which may indicate that this genotype increases the risk of pSLE, especially in boys, and also this genotype might increase the risk of central nervous system involvement. Therefore, eNOS gene SNP rs1808593 genotype may have an important role in predicting the occurrence of pSLE and central nervous system complications in pSLE.
July 2018
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